Recombinant Human Bone Morphogenetic Protein 4

• Cat.No.: BMP4-23H
• Product Overview: Recombinant human BMP4 is a protein expressed and purified fromhuman 293 cellsas a glycosylated homodimer with an apparent molecular mass of 34-38 kDa.

Specification

• Species: Human
• Source: HEK293
• Tag: Non
• Description: Bone Morphogenetic Protein 4 (BMP-4) is a polypeptide belonging to the TGF-β protein superfamily. BMP-4 is involved in bone and cartilage development; more specifically, in tooth and limb development fracture repair1. In human embryonic development, BMP-4 is a critical signaling molecule required for the early differentiation of the embryo and establishment of a dorsal-ventral axis 2,3. BMP-4 plays an important role in differentiation of overlying ectodermal tissue. Inhibition of the BMP-4 signal causes the ectoderm to differentiate into the neural plate. In cultured stem cells, BMP-4 plays a distinct role in mouse and human embryonic stem (ES) cells. BMP-4 supports LIF as a positive factor for mouse ES cell self-renewal4. In contrast, BMP-4 induces extra-embryonic trophoblast differentiation in human ES cells.
• Purity: Greater than 95 % by SDS-PAGE.
• Formulation: Lyophilized from sterile-filtered 50 mM NaOAc, pH 4.5 and 1 M NaCl containing no additives.
• Amino Acid Sequence: SPKHHSQRAR KKNKNCRRHS LYVDFSDVGW NDWIVAPPGY QAFY CHGDCPFPLAD HLNST NHA IVQTLVN SVN SSIPKAC CVPTELSAIS MLYLDEYDKV VLKNYQEMVV EGCGCR.
• Endotoxin Level: < 1 EU/μg of BMP-4 as determined by the LAL method.
• Biologic Activity: ED50 determined by its ability to induce alkaline phosphatase production by mouse chondrongenic ATDC-5 cells is 4.2 ng/ml.
• Storage And Handling: Stemfactor™ BMP-4, Human Recombinant, is stable in the lyophilized form for six months at -20°C to -80°C. Centrifuge briefly and reconstitute BMP-4 in 4 mM HCl to prepare a working stock solution of no less than 0.1 mg/ml. Reconstituted BMP-4 should be stored in working aliquots at -20°C for up to six months. Multiple freeze and thaw cycles will result in significant loss of activity.

Gene Information

• Gene Name: BMP4 bone morphogenetic protein 4 [ Homo sapiens ]
• Synonyms: ZYME; BMP2B; BMP2B1; DVR4; MCOPS6; BMP-2B; BMP-4; Bone morphogenetic protein 4 precursor; bone morphogenetic protein 2B; bone morphogenetic protein 4; BMP4
• Gene ID: 652
• mRNA Refseq: NM_001202
• Protein Refseq: NP_001193
• MIM: 112262
• UniProt ID: P12644
• Chromosome Location: 14q22-q23
• Function: cytokine activity. growth factor activity. heparin binding. protein binding. signal transducer activity