Recombinant Human BCL2L1 protein

• Cat.No.: BCL2L1-348H
• Product Overview: Recombinant Human BCL2L1 protein was expressed in Escherichia coli.

Specification

• Description: The protein encoded by this gene belongs to the BCL-2 protein family. BCL-2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. The proteins encoded by this gene are located at the outer mitochondrial membrane, and have been shown to regulate outer mitochondrial membrane channel (VDAC) opening. VDAC regulates mitochondrial membrane potential, and thus controls the production of reactive oxygen species and release of cytochrome C by mitochondria, both of which are the potent inducers of cell apoptosis. Alternative splicing results in multiple transcript variants encoding two different isoforms. The longer isoform acts as an apoptotic inhibitor and the shorter isoform acts as an apoptotic activator.
• Source: E.coli
• Species: Human
• Form: Lyophilized from a 0.2μm filtered concentrated solution in 20 mM Tris-HCl, pH 8.0, 5 % Trehalose.
• Molecular Mass: Approximately 23.7 kDa, a single non-glycosylated polypeptide chain containing 211 amino acids.
• Protein length: 211
• AA Sequence: SQSNRELVVDFLSYKLSQKGYSWSQ FSDVEENRTEAPEGTESEMETPSAI NGNPSWHLADSPAVNGATGHSSSLD AREVIPMAAVKQALREAGDEFELRY RRAFSDLTSQLHITPGTAYQSFEQV VNELFRDGVNWGRIVAFFSFGGALC VESVDKEMQVLVSRIAAWMATYLND HLEPWIQENGGWDTFVELYGNNAAA ESRKGQERFNR
• Endotoxin: Less than 0.1 EU/µg of rHuBcl-xL as determined by LAL method.
• Purity: >97% by SDS-PAGE and HPLC analysis.
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 centigrade as supplied. 1 month, 2 to 8 centigrade under sterile conditions after reconstitution. 3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
• Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤-20 centigrade. Further dilutions should be made in appropriate buffered solutions.
• Tag: Non

Gene Information

• Gene Name: BCL2L1
• Official Symbol: BCL2L1
• Synonyms: BCL2L1; BCL2-like 1; bcl-2-like protein 1; Bcl X; bcl xL; bcl xS; BCL2L; BCLX; PPP1R52; protein phosphatase 1; regulatory subunit 52; apoptosis regulator Bcl-X; protein phosphatase 1, regulatory subunit 52; BCLXL; BCLXS; Bcl-X; bcl-xL; bcl-xS; BCL-XL/S; DKFZp781P2092
• Gene ID: 598
• mRNA Refseq: NM_001191
• Protein Refseq: NP_001182
• MIM: 600039
• UniProt ID: Q07817