Recombinant Human Bcl2 Modifying Factor, T7-tagged

• Cat.No.: BMF-412H
• Product Overview: Recombinant human BMF, fused to T7-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography, 15.6 kDa (144aa)

Specification

• Species: Human
• Source: E.coli
• Tag: T7
• Description: Bcl2 modifying factor, also known as BMF belongs to the Bcl2 protein family of apoptosis mediators. Bmf is constitutively expressed in many tissues. This protein contains a single Bcl2 homology domain 3 (BH3), and has been shown to bind Bcl2 proteins and function as an apoptotic activator. Also, BMF is a key molecule for histone deacetylase (HDAC) inhibitors which alters the balance between acetylation and deacetylation, significantly increasing histone acetylation, while strongly inducing apoptosis in a variety of cancer cell types.
• Purity: > 90% by SDS - PAGE
• Sequences of amino acids: MASMTGGQQM GRGSHMEPSQ CVEELEDDVF QPEDGEPVTQ PGSLLSADLF AQSLLDCPLS RLQLFPLTHC CGPGLRPTSQ EDKATQTLSP ASPSQGVMLP CGVTEEPQRL FYAPAEPKSC VVADPPLPAQ PCFEWRREQE RGRP
• Concentration: 1 mg/ml (determined by Bradford assay)
• Form: Liquid. In 20 mM Tris-HCl buffer (pH 7.5) containing, 1mM DTT, 10% glycerol
• Endotoxin Level: < 1.0 EU per 1 μg of protein (determined by LAL method )
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.

Gene Information

• Gene Name: BMF Bcl2 modifying factor [ Homo sapiens ]
• Synonyms: BMF; Bcl2 modifying factor; FLJ00065; Bcl-2-modifying factor
• Gene ID: 90427
• mRNA Refseq: NM_001003940
• Protein Refseq: NP_001003940
• MIM: 606266
• UniProt ID: Q96LC9
• Chromosome Location: 15q14
• Pathway: Apoptosis
• Function: protein binding