Recombinant Human BACE2 protein, His-tagged

• Cat.No.: BACE2-1831H
• Product Overview: Recombinant Human BACE2 aa. (Met230-Phe396 (Accession # Q9Y5Z0)) fused with N-terminal His tag was produced in E. coli cells.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: Met230-Phe396
• Description: This gene encodes an integral membrane glycoprotein that functions as an aspartic protease. The encoded protein cleaves amyloid precursor protein into amyloid beta peptide, which is a critical step in the etiology of Alzheimer's disease and Down syndrome. The protein precursor is further processed into an active mature peptide. Alternative splicing results in multiple transcript variants.
• Form: Freeze-dried powder
• Molecular Mass: 19.9kDa as determined by SDS-PAGE reducing conditions.
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Purity: >97%
• Characteristic: The isoelectric point is 4.7.
• Applications: SDS-PAGE; WB; ELISA; IP.
• Stability: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage Buffer: Supplied as liquid form in Phosphate buffered saline(PBS), pH 7.4.
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.

Gene Information

• Gene Name: BACE2 beta-site APP-cleaving enzyme 2 [ Homo sapiens (human) ]
• Official Symbol: BACE2
• Synonyms: Ebi3; Epstein-Barr virus induced gene 3; EBI-3; IL-27; interleukin-27 subunit beta; IL-27 subunit beta; IL-27B; epstein-Barr virus-induced gene 3 protein homolog
• Gene ID: 25825
• mRNA Refseq: NM_012105.4
• Protein Refseq: NP_036237.2
• UniProt ID: Q9Y5Z0