Recombinant Human B4GALNT2 protein, His & T7-tagged

• Cat.No.: B4GALNT2-1690H
• Product Overview: Recombinant Human B4GALNT2 aa. (Thr324~Ala566 (Accession # Q8NHY0)) fused with N-terminal His & T7 tag was produced in E. coli cells.

Specification

• Description: B4GALNT2 catalyzes the last step in the biosynthesis of the human Sd(a) antigen through the addition of an N-acetylgalactosamine residue via a beta-1,4 linkage to a subterminal galactose residue substituted with an alpha-2,3-linked sialic acid. B4GALNT2 also catalyzes the last step in the biosynthesis of the Cad antigen (Montiel et al., 2003 [PubMed 12678917]).
• Source: E. coli
• Species: Human
• Tag: His&T7
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 31.3kDa
• Protein length: Thr324~Ala566
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Purity: >95%
• Applications: SDS-PAGE; WB; ELISA; IP.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage buffer: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Isoelectric Point: 6.2

Gene Information

• Gene Name: B4GALNT2 beta-1,4-N-acetyl-galactosaminyltransferase 2 [ Homo sapiens (human) ]
• Official Symbol: B4GALNT2
• Synonyms: B4GALNT2; beta-1,4-N-acetyl-galactosaminyltransferase 2; B4GALT; GALGT2; beta-1,4 N-acetylgalactosaminyltransferase 2; UDP-GalNAc:Neu5Aca2-3Galb-R b1,4-N-acetylgalactosaminyltransferase; UDP-GalNAc:Neu5Acalpha2-3Galbeta-R beta1,4-N-acetylgalactosaminyltransferase; beta 1,4 N-acetylgalactosaminyltransferase/betal,4 GalNAcT/Sda-GalNAcT; beta-1,4-N-acetyl-galactosaminyl transferase 2; beta-4-N-acetylgalactosaminyltransferase; sd(a) beta-1,4-GalNAc transferase; sda beta-1,4-GalNAc transferase
• Gene ID: 124872
• mRNA Refseq: NM_001159387.1
• Protein Refseq: NP_001152859.1
• UniProt ID: Q8NHY0