Recombinant Human B-Cell CLL/Lymphoma 2, His-tagged

• Cat.No.: BCL2-5289H
• Product Overview: Recombinant human BCL2with His Tag was expressed in E. coli.

Specification

• Cat. No.: BCL2-5289H
• Description: Recombinantfull length human BCL2 is a single, non-glycosylated polypeptide chaincontaining amino acids 1-206. The wild type BCL2 is missing 12 amino acidsfrom the C-terminus. The C-terminus is fused to a His-Tag. The C-terminushis-tag mimics the deleted C-terminus membrane domain thus maintaining itsbiological activity.
• Source: E. coli
• Species: Human
• Form: Lyophilized from asterile filtered solution containing 10 mM Tris-HCl, pH 8.0 + 1 mM EDTA + 250mM NaCl.
• Purity: > 95% asdetermined by RP-HPLC and SDS-PAGE analyses.
• Endotoxin Level: < 0.1 ng/µg ofBCL2
• Reconstitution: Centrifuge vialprior to opening. Suspend BCL2 in 100 µl of 0.5M Acetic acid, overnight at 2-4°C. Dilute 10 fold into selected buffer system. Because BCL2 hasa tendency to form intramolecular disulfide bond, 5 mM DTT is recommended inassay buffer. When running SDS-PAGE gel, 10 mM DTT is recommended.
• Storage: Store lyophilizedprotein at -20°C to -80°C. Reconstituted protein is stable for 1 week at2-4°C. For long term storage, aliquot and store at -20°C to -80°C with acarrier protein (0.1% HSA or BSA) as a stabilizer. Please note that theaddition of any carrier protein into this product may produce unwantedendotoxin. This depends upon the particular application employed. Avoidrepeated freeze-thaw cycles.
• OfficialSymbol: BCL2

Gene Information

• Gene Name: BCL2 B-cell CLL/lymphoma 2 [ Homosapiens ]
• Synonyms: BCL2; B-cellCLL/lymphoma 2; Bcl-2; PPP1R50; apoptosisregulator Bcl-2; protein phosphatase 1, regulatory subunit 50
• Gene ID: 596
• mRNA Refseq: NM_000633
• Protein Refseq: NP_000624
• MIM: 151430
• UniProt ID: P10415
• Chromosome Location: 18q21.33; 18q21.3
• Pathway: ATF-2 transcriptionfactor network; Activation of BAD and translocation to mitochondria;Activation of BH3-only proteins; Amyotrophic lateral sclerosis (ALS);Apoptosis; B Cell Receptor Signaling Pathway; BH3-only proteins associatewith and inactivate anti-apoptotic BCL-2 members; Caspase cascade inapoptosis; Cholinergic synapse; Colorectal cancer; RXR and RARheterodimerization with other nuclear receptor; Small cell lung cancer; TheNLRP1 inflammasome; Toxoplasmosis; Tuberculosis
• Function: BH3 domain binding;channel activity; identical protein binding; protease binding; proteinbinding; protein heterodimerization activity; protein homodimerizationactivity; protein phosphatase 2A binding; sequence-specific DNA binding;transcription factor binding; ubiquitin protein ligase binding