Recombinant Human Aurora Kinase A, His-tagged

• Cat.No.: AURKA-106H
• Product Overview: Recombinant human Aurora-A fused to His-tag at N-terminus, was expressed inE.coliand purified by using conventional chromatography. MW =47.9 kDa (423aa).

Specification

• Cat. No.: AURKA-106H
• Description: Aurora Kinase A is a member of a family of mitotic serine/threonine kinases. It is implicated with important processes during mitosis and meiosis whose proper function is integral for healthy cell proliferation. Aurora kinase A is highly expressed in testis and weakly in skeletal muscle, thymus and spleen. Also this protein plays a crucial role in tumourigenesis and is overexpressed in many kinds of cancers.
• Source: E.coli.
• Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MDRSKENCIS GPVKATAPVG GPKRVLVTQQ FPCQNPLPVN SGQAQRVLCP SNSSQRIPLQ AQKLVSSHKP VQNQKQKQLQ ATSVPHPVSR PLNNTQKSKQ PLPSAPENNP EEELASKQKN EESKKRQWAL EDFEIGRPLG KGKFGNVYLA REKQSKFILA LKVLFKAQLE KAGVEHQLRR EVEIQSHLRH PNILRLYGYF HDATRVYLIL EYAPLGTVYR ELQKLSKFDE QRTATYITEL ANALSYCHSK RVIHRDIKPE NLLLGSAGEL KIADFGWSVH APSSRRTTLC GTLDYLPPEM IEGRMHDEKV DLWSLGVLCY EFLVGKPPFE ANTYQETYKR ISRVEFTFPD FVTEGARDLI SRLLKHNPSQ RPMLREVLEH PWITANSSKP SNCQNKESAS KQS.
• Form: Liquid. In 20 mM Tris-HCl buffer (pH 8.0) containing 0.5mM DTT, 100mM NaCl, 0.1mM EDTA, 0.1mM EGTA, 0.1mM PMSF, 20% glycerol.
• Purity: > 95% by SDS – PAGE.
• Concentration: 0.5 mg/ml (determined by Bradford assay).
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.

Gene Information

• Gene Name: AURKA aurora kinase A [ Homo sapiens ]
• Synonyms: AURKA ; aurora kinase A; AIK; ARK1; AURA; BTAK; STK6; STK7; STK15; AURORA2; MGC34538; OTTHUMP00000031344; OTTHUMP00000166071; IPL1-related kinase; aurora-related kinase 1; aurora/IPL1-like kinase; serine/threonine kinase 6; breast-tumor-amplified kinase; serine/threonine protein kinase 15; EC 2.7.11.1; AurA; Aurora kinase A; Aurora-A; Serine/threonine kinase 15; hARK1; Breast tumor-amplified kinase; OTTHUMP00000031340; OTTHUMP00000031342; OTTHUMP00000031343; OTTHUMP00000031345; Breast tumor-amplified kinase 3; serine/threonine protein kinase 6
• Gene ID: 6790
• mRNA Refseq: NM_003600
• Protein Refseq: NP_003591
• MIM: 603072
• UniProt ID: O14965
• Chromosome Location: 20q13
• Pathway: Cell Cycle, Mitotic
• Function: ATP binding; nucleotide binding; protein binding; protein binding; protein serine/threonine kinase activity; transferase activity; ubiquitin protein ligase binding

Reviews

12/08/2022

AURKA's exceptional stability allows for its reliable use in a range of applications, making it a top choice for researchers.

02/10/2022

This protein's exceptional activity allows for its reliable use in a range of applications, making it a top choice for researchers.

10/24/2020

Due to the extensive storage conditions of proteins, the experimental process is more stable and reliable, which provides a strong guarantee for scientific research.

Q&As

How does AURKA interact with cell cycle regulators?

AURKA protein interacts with a variety of cell cycle regulatory proteins, including Cdk1, Plk1, TPX2, etc., to regulate the progression of cell cycle.

What is the role of AURKA protein in the cell cycle?

The protein plays an important role in the G2 and M phases of the cell cycle and is involved in the regulation of nuclear division, chromosome segregation, and cell mitosis.

Is AURKA protein involved in apoptosis and DNA damage repair?

AURKA protein may be involved in the regulation of apoptosis and DNA damage repair, but the specific mechanism needs to be further studied.

What is the regulatory mechanism of AURKA protein activity?

The activity is regulated by a variety of factors, including phosphorylation modification, protein interaction and cell cycle regulation.

Where are the cells of AURKA protein?

It is mainly localized in the cytoplasm and nucleus and is involved in cell cycle regulation and cell division processes.

What is the role of AURKA protein in tumorigenesis?

This protein plays an important role in tumorigenesis, and its overexpression or mutation may lead to abnormal cell proliferation, chromosome instability and tumorigenesis.