Recombinant Human ATP6V1E1, GST-tagged

• Cat.No.: ATP6V1E1-10046H
• Product Overview: Recombinant Human ATP6V1E1 protein, fused to GST-tag, was expressed in E.coli and purified by GSH-sepharose.

Specification

• Description: This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A, three B, and two G subunits, as well as a C, D, E, F, and H subunit. The V1 domain contains the ATP catalytic site. This gene encodes alternate transcriptional splice variants, encoding different V1 domain E subunit isoforms. Pseudogenes for this gene have been found in the genome.
• Source: E.coli
• Species: Human
• Tag: GST
• Protein length: 1-226a.a.
• Storage: The protein is stored in PBS buffer at -20℃. Avoid repeated freezing and thawing cycles.
• Storage Buffer: 1M PBS (58mM Na2HPO4,17mM NaH2PO4, 68mM NaCl, pH8. ) added with 100mM GSH and 1% Triton X-100,15%glycerol.

Gene Information

• Gene Name: ATP6V1E1 ATPase, H+ transporting, lysosomal 31kDa, V1 subunit E1 [ Homo sapiens ]
• Official Symbol: ATP6V1E1
• Synonyms: ATP6V1E1; ATPase, H+ transporting, lysosomal 31kDa, V1 subunit E1; ATP6E, ATP6V1E, ATPase, H+ transporting, lysosomal (vacuolar proton pump) 31kD , ATPase, H+ transporting, lysosomal 31kDa, V1 subunit E isoform 1; V-type proton ATPase subunit E 1; ATP6E2; P31; Vma4; V-ATPase, subunit E; V-ATPase subunit E 1; V-ATPase 31 kDa subunit; vacuolar proton pump subunit E 1; H+-transporting ATP synthase chain E, vacuolar; H(+)-transporting two-sector ATPase, 31kDa subunit; ATP6E; ATP6V1E
• Gene ID: 529
• mRNA Refseq: NM_001039366
• Protein Refseq: NP_001034455
• MIM: 108746
• UniProt ID: P36543
• Chromosome Location: 22q11.2
• Pathway: Collecting duct acid secretion, organism-specific biosystem; Collecting duct acid secretion, conserved biosystem; Epithelial cell signaling in Helicobacter pylori infection, organism-specific biosystem; Epithelial cell signaling in Helicobacter pylori infection, conserved biosystem; Insulin receptor recycling, organism-specific biosystem; Iron uptake and transport, organism-specific biosystem; Metabolic pathways, organism-specific biosystem
• Function: hydrogen-exporting ATPase activity, phosphorylative mechanism; hydrolase activity; protein binding; proton-transporting ATPase activity, rotational mechanism