Recombinant Human ATP1b3, His-tagged, T7 tagged

• Cat.No.: ATP1b3-3767H
• Product Overview: ATPase, Na+/K+ Transporting Beta 3 Polypeptide (ATP1b3)

Specification

• Species: Human
• Source: E.coli
• Tag: His&T7
• Protein Length: 279
• Description: The protein encoded by this gene belongs to the family of Na+/K+ and H+/K+ ATPases beta chain proteins, and to the subfamily of Na+/K+ -ATPases. Na+/K+ -ATPase is an integral membrane protein responsible for establishing and maintaining the electrochemical gradients of Na and K ions across the plasma membrane. These gradients are essential for osmoregulation, for sodium-coupled transport of a variety of organic and inorganic molecules, and for electrical excitability of nerve and muscle. This enzyme is composed of two subunits, a large catalytic subunit (alpha) and a smaller glycoprotein subunit (beta). The beta subunit regulates, through assembly of alpha/beta heterodimers, the number of sodium pumps transported to the plasma membrane. The glycoprotein subunit of Na+/K+ -ATPase is encoded by multiple genes. This gene encodes a beta 3 subunit. This gene encodes a beta 3 subunit. A pseudogene exists for this gene, and it is located on chromosome 2.
• Form: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Molecular Mass: 27.7kDa
• Endotoxin: <1.0eu per 1ug (determined by the lal</1.0eu
• Purity: >95%
• Applications: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Notes: Small volumes of ATP1b3 recombinant protein may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Warning: This product is for research use only. Not for use in diagnostic or therapeutic procedures.

Gene Information

• Gene Name: ATP1B3 ATPase, Na+/K+ transporting, beta 3 polypeptide [ Homo sapiens ]
• Official Symbol: ATP1b3
• Synonyms: ATP1B3; ATPase, Na+/K+ transporting, beta 3 polypeptide; sodium/potassium-transporting ATPase subunit beta-3; CD298; FLJ29027; Na, K-ATPase beta-3 polypeptide; sodium/potassium-dependent ATPase beta-3 subunit; sodium/potassium-dependent ATPase subunit beta-3; sodium/potassium-transporting ATPase beta-3 chain; ATPB-3
• Gene ID: 483
• mRNA Refseq: NM_001679
• Protein Refseq: NP_001670
• MIM: 601867
• UniProt ID: P54709
• Chromosome Location: 3q23
• Pathway: Aldosterone-regulated sodium reabsorption, organism-specific biosystem; Aldosterone-regulated sodium reabsorption, conserved biosystem; Basigin interactions, organism-specific biosystem; Bile secretion, organism-specific biosystem; Bile secretion, conserv
• Function: sodium:potassium-exchanging ATPase activity