Recombinant Human ARF6 protein, His-tagged

• Cat.No.: ARF6-26533TH
• Product Overview: Recombinant Human ARF6(1-175 aa) fused with His tag was expressed in E. coli.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 1-175 a.a.
• Description: ADP-ribosylation factor 6, also known as ARF6, is a member of the ADP ribosylation factor family of GTP-binding proteins. It is localized to the plasma membrane, and regulates vesicular trafficking, remodelling of membrane lipids, and signaling pathways that lead to actin remodeling. Additionally, ARF6 play a central role in maintenance of organelle integrity, assembly of coat proteins and activation of phospholipase D. Recombinant human ARF6 protein , fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
• Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 2mM DTT, 20% glycerol, 0.2mM PMSF
• Molecular Mass: 22.2 kDa (195aa) confirmed by MALDI-TOF
• AA Sequence: MGSSHHHHHH SSGLVPRGSHRPLWRHYYTG TQGLIFVVDC ADRDRIDEAR QELHRIINDR EMRDAIILIF ANKQDLPDAM KPHEIQEKLG LTRIRDRNWY VQPSCATSGDMGKVLSKIFG NKEMRILMLG LDAAGKTTIL YKLKLGQSVT TIPTVGFNVE TVTYKNVKFN VWDVGGQDKI GLYEGLTWLT SNYKS
• Purity: > 95% by SDS - PAGE
• Storage: Can be stored at +4 centigrade short term (1-2 weeks). For long term storage, aliquot and store at -20 centigrade or -70 centigrade. Avoid repeated freezing and thawing cycles.
• Concentration: 1 mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: ARF6 ADP-ribosylation factor 6 [ Homo sapiens ]
• Official Symbol: ARF6
• Synonyms: ARF6; ADP-ribosylation factor 6; DKFZp564M0264
• Gene ID: 382
• mRNA Refseq: NM_001663
• Protein Refseq: NP_001654
• MIM: 600464
• UniProt ID: P62330
• Chromosome Location: 14q21.3
• Pathway: Arf6 downstream pathway, organism-specific biosystem; Arf6 signaling events, organism-specific biosystem; Arf6 trafficking events, organism-specific biosystem; Class I PI3K signaling events, organism-specific biosystem; E-cadherin signaling in the nascent adherens junction, organism-specific biosystem; Endocytosis, organism-specific biosystem; Endocytosis, conserved biosystem
• Function: GTP binding; GTPase activity; nucleotide binding; protein binding; thioesterase binding