Active Recombinant Human ANGPTL7 Protein, mIgG2aFc-Avi-tagged, Biotinylated

• Cat.No.: ANGPTL7-162H
• Product Overview: Biotinylated Human ANGPTL7, Mouse IgG2a Fc Tag, Avi Tag is expressed from HEK293 cells. It contains AA Gln 27 - Pro 346 (Accession # O43827-1). This protein carries a mouse IgG2a Fc tag at the C-terminus, followed by an Avi tag.

Specification

• Species: Human
• Source: HEK293
• Tag: Avi&mIgG2a
• Protein Length: 27-346 a.a.
• Predicted N Terminal: Gln 27
• Form: Lyophilized from 0.22 um filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5, 10% trehalose.
• Bio-activity: Immobilized Human LILRB4, Fc Tag at 1 ug/mL (100 uL/well) can bind Biotinylated Human ANGPTL7, Mouse IgG2a Fc Tag, Avi Tag with a linear range of 0.078-2.5 ug/mL. Immobilized Human LILRB2, Fc Tag at 1 ug/mL (100 uL/well) can bind Biotinylated Human ANGPTL7, Mouse IgG2a Fc Tag, Avi Tag with a linear range of 0.078-1.25 ug/mL. Immobilized Human LILRA3, Fc Tag at 1 ug/mL (100 uL/well) can bind Biotinylated Human ANGPTL7, Mouse IgG2a Fc Tag, Avi Tag with a linear range of 0.078-5 ug/mL. Immobilized Human LILRB5, His Tag at 5 ug/mL (100 uL/well) can bind Biotinylated Human ANGPTL7, Mouse IgG2a Fc Tag, Avi Tag with a linear range of 20-625 ug/mL.
• Molecular Mass: The protein has a calculated MW of 66.5 kDa. The protein migrates as 66 kDa and 75-95 kDa under reducing condition (SDS-PAGE) due to glycosylation.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: >90% as determined by SDS-PAGE.
• Characteristic: Biotinylation of this product is performed using Avitag technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
• Stability: Samples are stable for up to twelve months from date of receipt at -70ºC.
• Storage: For long term storage, the product should be stored at lyophilized state at -20ºC or lower. Please avoid repeated freeze-thaw cycles. No activity loss is observed after storage at: 4-8ºC for 12 months in lyophilized state; -70ºC for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.

Gene Information

• Gene Name: ANGPTL7 angiopoietin-like 7 [ Homo sapiens ]
• Official Symbol: ANGPTL7
• Synonyms: ANGPTL7; angiopoietin-like 7; angiopoietin-related protein 7; AngX; CDT6; angiopoietin-like protein 7; angiopoietin-like factor (CDT6); cornea-derived transcript 6 protein; dJ647M16.1; RP4-647M16.2
• Gene ID: 10218
• mRNA Refseq: NM_021146
• Protein Refseq: NP_066969
• UniProt ID: O43827