Recombinant Human ANGPT1, FLAG-tagged

• Cat.No.: ANGPT1-27317TH
• Product Overview: Recombinant fragment corresponding to amino acids 20-498 of Human Angiopoietin 1 with an N terminual DDDDK tag; MWt 57 kDa .

Specification

• Description: Angiopoietins are proteins with important roles in vascular development and angiogenesis. All angiopoietins bind with similar affinity to an endothelial cell-specific tyrosine-protein kinase receptor. The protein encoded by this gene is a secreted glycoprotein that activates the receptor by inducing its tyrosine phosphorylation. It plays a critical role in mediating reciprocal interactions between the endothelium and surrounding matrix and mesenchyme and inhibits endothelial permeability. The protein also contributes to blood vessel maturation and stability, and may be involved in early development of the heart. Alternative splicing results in multiple transcript variants encoding distinct isoforms.
• Protein length: 479 amino acids
• Conjugation: FLAG
• Molecular Weight: 57.000kDa inclusive of tags
• Source: E. coli
• Biological activity: Induces phosphorylation of Tie-2 in primary cultured human vascular endothelial cells (HUVECs). Binds to Human Tie-2. The protein forms mainly tetrameric structures.
• Form: Liquid
• Purity: >90% by SDS-PAGE
• Storage buffer: Preservative: NoneConstituents: 0.02% CHAPS, PBS
• Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
• Sequence Similarities: Contains 1 fibrinogen C-terminal domain.

Gene Information

• Gene Name: ANGPT1 angiopoietin 1 [ Homo sapiens ]
• Official Symbol: ANGPT1
• Synonyms: ANGPT1; angiopoietin 1; angiopoietin-1; Ang1; KIAA0003
• Gene ID: 284
• mRNA Refseq: NM_001146
• Protein Refseq: NP_001137
• MIM: 601667
• Uniprot ID: Q15389
• Chromosome Location: 8q23.1
• Pathway: Angiopoietin receptor Tie2-mediated signaling, organism-specific biosystem; Cell surface interactions at the vascular wall, organism-specific biosystem; Hemostasis, organism-specific biosystem; Rheumatoid arthritis, organism-specific biosystem; Rheumatoid arthritis, conserved biosystem
• Function: receptor tyrosine kinase binding