Recombinant human aminopeptidase puromycin sensitive protein, His tagged

• Cat.No.: NPEPPS-07H
• Product Overview: Recombinant human NPEPPS (1-919aa), fused to His-tag at C-terminus, was expressed in HEK293 and purifed by using conventional chromatography techniques.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: 1-919aa
• Description: This gene encodes the puromycin-sensitive aminopeptidase, a zinc metallopeptidase which hydrolyzes amino acids from the N-terminus of its substrate. The protein has been localized to both the cytoplasm and to cellular membranes. This enzyme degrades enkaphalins in the brain, and studies in mouse suggest that it is involved in proteolytic events regulating the cell cycle.
• Tag: C-His
• Form: Liquid
• Bio-activity: Specific activity is > 800 pmol/min/μg, and is defined as the amount of enzyme that cleaves 1 pmole of H-Leu-AMC per minute at pH7.0 at 37 centigrade.
• Molecular Mass: 104 kDa
• AA Sequence: MWLAAAAPSLARRLLFLGPPPPPLLLLVFSRSSRRRLHSLGLAAMPEKRPFERLPADVSPINYSLCLKPDLLDFTFEGKLEAAAQVRQATNQIVMNCADIDIITASYAPEGDEEIHATGFNYQNEDEKVTLSFPSTLQTGTGTLKIDFVGELNDKMKGFYRSKYTTPSGEVRYAAVTQFEATDARRAFPCWDEPAIKATFDISLVVPKDRVALSNMNVIDRKPYPDDENLVEVKFARTPVMSTYLVAFVVGEYDFVETRSKDGVCVRVYTPVGKAEQGKFALEVAAKTLPFYKDYFNVPYPLPKIDLIAIADFAAGAMENGLVTYRETALLIDPKNSCSSSRQWVALVVGHELAHQWFGNLVTMEWWTHLWLNEGFASWIEYLCVDHCFPEYDIWTQFVSADYTRAQELDALDNSHPIEVSVGHPSEVDEIFDAISYSKGASVIRMLHDYIGDKDFKKGMNMYLTKFQQKNAATEDLWESLENASGKPIAAVMNTWTKQMGFPLIYVEAEQVEDDRLLRLSQKKFCAGGSYVGEDCPQWMVPITISTSEDPNQAKLKILMDKPEMNVVLKNVKPDQWVKLNLGTVGFYRTQYSSAMLESLLPGIRDLSLPPVDRLGLQNDLFSLARAGIISTVEVLKVMEAFVNEPNYTVWSDLSCNLGILSTLLSHTDFYEEIQEFVKDVFSPIGERLGWDPKPGEGHLDALLRGLVLGKLGKAGHKATLEEARRRFKDHVEGKQILSADLRSPVYLTVLKHGDGTTLDIMLKLHKQADMQEEKNRIERVLGATLLPDLIQKVLTFALSEEVRPQDTVSVIGGVAGGSKHGRKAAWKFIKDNWEELYNRYQGGFLISRLIKLSVEGFAVDKMAGEVKAFFESHPAPSAERTIQQCCENILLNAAWLKRDAESIHQYLLQRKASPPTV
• Endotoxin: < 1 EU/μg of protein (determined by LAL method)
• Purity: > 85% by SDS-PAGE
• Applications: SDS-PAGE, Enzyme Activity
• Notes: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.
• Storage: Can be stored at +2 to +8 centigrade for 1 week. For long term storage, aliquot and store at -20 to -80 centigrade. Avoid repeated freezing and thawing cycles.
• Storage Buffer: Phosphate-Buffered Saline (pH 7.4) containing 20% glycerol
• Concentration: 0.25 mg/mL (determined by Absorbance at 280nm)
• References: 1. Kudo LC, et al. (2011) Hum Mol Genet. 20:1820-1833.
  2. Jones RT, et al. (2024) Cancer Res. 84:1699-1718.
  3. Lenskaya V, et al. (2024) J Cutan Pathol. 51:419-423.

Gene Information

• Gene Name: NPEPPS aminopeptidase puromycin sensitive [ Homo sapiens (human) ]
• Official Symbol: NPEPPS
• Synonyms: NPEPPS; aminopeptidase puromycin sensitive; PSA; AAP-S; MP100; puromycin-sensitive aminopeptidase; cytosol alanyl aminopeptidase; metalloproteinase MP100; EC 3.4.11.14
• Gene ID: 9520
• mRNA Refseq: NM_006310
• Protein Refseq: NP_006301
• MIM: 606793
• UniProt ID: P55786