Recombinant Human alveolar soft part sarcoma chromosome region, candidate 1, His-tagged

• Cat.No.: ASPSCR1-211H
• Product Overview: ASPSCR1, 1-553aa, Human, His tag, E.coli

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 1-553 a.a.
• Description: ASPSCR1 contains a UBX domain and interacts with glucose transporter type 4 (GLUT4). This protein is a tether, which sequesters the GLUT4 in intracellular vesicles in muscle and fat cells in the absence of insulin, and redistributes the GLUT4 to the plasma membrane within minutes of insulin stimulation. Translocation t(X;17)(p11;q25) of this gene with transcription factor TFE3 gene results in a ASPSCR1-TFE3 fusion protein in alveolar soft part sarcoma and in renal cell carcinomas. Recombinant human ASPSCR1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
• Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol, 1mM DTT.
• Molecular Mass: 62.6kDa (576aa)
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSMAAPAGG GGSAVSVLAP NGRRHTVKVT PSTVLLQVLE DTCRRQDFNP CEYDLKFQRS VLDLSLQWRF ANLPNNAKLE MVPASRSREG PENMVRIALQ LDDGSRLQDS FCSGQTLWEL LSHFPQIREC LQHPGGATPV CVYTRDEVTG EAALRGTTLQ SLGLTGGSAT IRFVMKCYDP VGKTPGSLGS SASAGQAAAS APLPLESGEL SRGDLSRPED ADTSGPCCEH TQEKQSTRAP AAAPFVPFSG GGQRLGGPPG PTRPLTSSSA KLPKSLSSPG GPSKPKKSKS GQDPQQEQEQ ERERDPQQEQ ERERPVDREP VDREPVVCHP DLEERLQAWP AELPDEFFEL TVDDVRRRLA QLKSERKRLE EAPLVTKAFR EAQIKEKLER YPKVALRVLF PDRYVLQGFF RPSETVGDLR DFVRSHLGNP ELSFYLFITP PKTVLDDHTQ TLFQANLFPA ALVHLGAEEP AGVYLEPGLL EHAISPSAAD VLVARYMSRA AGSPSPLPAP DPAPKSEPAA EEGALVPPEP IPGTAQPVKR SLGKVPKWLK LPASKR
• Purity: >85% by SDS - PAGE
• Storage: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
• Concentration: 0.25 mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: ASPSCR1 alveolar soft part sarcoma chromosome region, candidate 1 [ Homo sapiens ]
• Official Symbol: ASPSCR1
• Synonyms: ASPSCR1; alveolar soft part sarcoma chromosome region, candidate 1; tether containing UBX domain for GLUT4; ASPL; ASPS; UBX domain protein 9; UBXD9; UBXN9; UBX domain-containing protein 9; alveolar soft part sarcoma locus; renal cell carcinoma, papillary, 17; renal papillary cell carcinoma protein 17; renal cell carcinoma gene on chromosome 17; alveolar soft part sarcoma chromosomal region candidate gene 1 protein; TUG; RCC17; ASPCR1; FLJ45380
• Gene ID: 79058
• mRNA Refseq: NM_001251888
• Protein Refseq: NP_001238817
• MIM: 606236
• UniProt ID: Q9BZE9
• Chromosome Location: 17q25
• Pathway: Transcriptional misregulation in cancer, organism-specific biosystem; Transcriptional misregulation in cancer, conserved biosystem
• Function: molecular_function; protein binding