Recombinant Human Allograft Inflammatory Factor 1, His-tagged

• Cat.No.: AIF1-6900H
• Product Overview: Recombinant human AIF1, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques. MW: 18.9 kDa.

Specification

• Description: AIF1 (Allograft inflammatory factor 1) is induced by cytokines and interferon. This protein is thought to be involved in negative regulation of growth of vascular smooth muscle cells, which contributes to the anti-inflammatory response to vessel wall trauma. AIF1 is a cytoplasm, calcium-binding protein whose expression in transplanted human hearts correlates with rejection and development of coronary artery vasculopathy (CAV).
• Source: E. coli
• Species: Human
• Tag: His
• Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 2mM DTT, 20% glycerol, 200mM NaCl.
• Molecular Mass: 18.9 kDa (167aa), confirmed by MALDI-TOF
• Protein length: 1-147aa
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MSQTRDLQGG KAFGLLKAQQ EERLDEINKQ FLDDPKYSSD EDLPSKLEGF KEKYMEFDLN GNGDIDIMSL KRMLEKLGVP KTHLELKKLI GEVSSGSGET FSYPDFLRMM LGKRSAILKM ILMYEEKARE KEKPTGPPAK KAISELP
• Purity: >95% by SDS-PAGE
• Applications: SDS-PAGE
• Storage: Can be stored at 4℃ short term (1-2 weeks). For long term storage, aliquot and store at -20℃ or -70℃. Avoid repeated freezing and thawing cycles.
• Concentration: 0.5 mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: AIF1 allograft inflammatory factor 1 [ Homo sapiens ]
• Official Symbol: AIF1
• Synonyms: AIF1; allograft inflammatory factor 1; AIF 1; Em:AF129756.17; IBA1; interferon gamma responsive transcript; ionized calcium binding adapter molecule 1; IRT 1; protein G1; ionized calcium-binding adapter molecule 1; allograft inflammatory factor-1 splice variant Hara-1; IRT1; AIF-1; IRT-1
• Gene ID: 199
• mRNA Refseq: NM_001623
• Protein Refseq: NP_001614
• MIM: 601833
• UniProt ID: P55008
• Chromosome Location: 6p21.3
• Function: actin filament binding; calcium ion binding; calcium ion binding