Recombinant Human AKR1C4, His-tagged

• Cat.No.: AKR1C4-29344TH
• Product Overview: Recombinant full length Human HSD3a with an N terminal His tag; 343 amino acids including tag, MWt 39.2kDa.

Specification

• Description: This gene encodes a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. These enzymes catalyze the conversion of aldehydes and ketones to their corresponding alcohols by utilizing NADH and/or NADPH as cofactors. The enzymes display overlapping but distinct substrate specificity. This enzyme catalyzes the bioreduction of chlordecone, a toxic organochlorine pesticide, to chlordecone alcohol in liver. This gene shares high sequence identity with three other gene members and is clustered with those three genes at chromosome 10p15-p14.
• Protein length: 343 amino acids
• Conjugation: HIS
• Molecular Weight: 39.200kDa inclusive of tags
• Source: E. coli
• Tissue specificity: Liver specific.
• Biological activity: Specific activity: approximately >0.6 units/mg. Enzymatic activity was confirmed by measuring the amount of enzyme catalyzing the oxidation of 1 micromole NADPH per minute at 25°C. Specific activity was expressed as units/mg protein.
• Form: Liquid
• Purity: >90% by SDS-PAGE
• Storage buffer: Preservative: NoneConstituents: 20% Glycerol, 0.58% Sodium chloride, 0.32% Tris HCl, 0.02% DTT, pH 8.0
• Storage: Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Sequences of amino acids: MGSSHHHHHHSSGLVPRGSHMDPKYQRVELNDGHFMPVLGFGTYAPPEVPRNRAVEVTKLAIEAGFRHIDSAYLYNNEEQVGLAIRSKIADGSVKREDIFYTSKLWCTFFQPQMVQPALESSLKKLQLDYVDLYLLHFPMALKPGETPLPKDENGKVIFDTVDLSATWEVMEKCKDAGLAKSIGVSNFNCRQLEMILNKPGLKYKPVCNQVECHPYLNQSKLLDFCKSKDIVLVAHSALGTQRHKLWVDPNSPVLLEDPVLCALAKKHKRTPALIALRYQLQRGVVVLAKSYNEQRIRENIQVFEFQLTSEDMKVLDGLNRNYRYVVMDFLMDHPDYPFSDEY
• Sequence Similarities: Belongs to the aldo/keto reductase family.

Gene Information

• Gene Name: AKR1C4 aldo-keto reductase family 1, member C4 (chlordecone reductase; 3-alpha hydroxysteroid dehydrogenase, type I; dihydrodiol dehydrogenase 4) [ Homo sapiens ]
• Official Symbol: AKR1C4
• Synonyms: AKR1C4; aldo-keto reductase family 1, member C4 (chlordecone reductase; 3-alpha hydroxysteroid dehydrogenase, type I; dihydrodiol dehydrogenase 4); CHDR; aldo-keto reductase family 1 member C4; 3 alpha HSD; C11; CDR; DD4; HAKRA; MGC22581
• Gene ID: 1109
• mRNA Refseq: NM_001818
• Protein Refseq: NP_001809
• MIM: 600451
• Uniprot ID: P17516
• Chromosome Location: 10p15.1
• Pathway: Bile acid and bile salt metabolism, organism-specific biosystem; Bile acid biosynthesis, cholesterol => chenodeoxycholate, organism-specific biosystem; Bile acid biosynthesis, cholesterol => chenodeoxycholate, conserved biosystem
• Function: aldo-keto reductase (NADP) activity; androsterone dehydrogenase (B-specific) activity; bile acid transmembrane transporter activity; chlordecone reductase activity; electron carrier activity