Recombinant Human AKR1B10, His-tagged

• Cat.No.: AKR1B10-2764H
• Product Overview: Recombinant human ARL1 protein, fused to His-tag at N-terminus, was expressed inE.coliand purified by using conventional chromatography techniques.

Specification

• Cat. No.: AKR1B10-2764H
• Description: ADP-ribosylation factor-like protein 1(ARL1) belongs to the ARL (ADP-ribosylation factor-like) family of proteins, which are structurally related to ADP-ribosylation factors (ARFs). ARFs, described as activators of cholera toxin (CT) ADP-ribosyltransferase activity, regulate intracellular vesicular membrane trafficking, and stimulate a phospholipase D (PLD) isoform. Although, ARL proteins were initially thought not to activate CT or PLD, later work showed that they are weak stimulators of PLD and CT in a phospholipid dependent manner.
• Source: E.coli.
• Purity: > 95% by SDS – PAGE.
• Sequences: MGSSHHHHHH SSGLVPRGSH MGGFFSSIFS SLFGTREMRI LILGLDGAGK TTILYRLQVG EVVTTIPTIG FNVETVTYKN LKFQVWDLGG QTSIRPYWRC YYSNTDAVIY VVDSCDRDRI GISKSELVAM LEEEELRKAI LVVFANKQDM EQAMTSSEMA NSLGLPALKD RKWQIFKTSA TKGTGLDEAM EWLVETLKSR Q.
• Molecular Weight: 22.5kDa (201aa)confirmed by MALDI-TOF.
• Form: Liquid. 20mM Tris-HCl buffer (pH8.0) containing 40% glycerol, 100mM NaCl, 2mM DTT.
• Concentration: 0.25mg/ml (determined by Bradford assay).
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.

Gene Information

• Gene Name: AKR1B10 aldo-keto reductase family 1, member B10 (aldose reductase) [ Homo sapiens ]
• Synonyms:
  AKR1B10; aldo-keto reductase family 1, member B10 (aldose reductase); HIS; HSI; ARL1; ARL-1; ALDRLn; AKR1B11; AKR1B12; MGC14103; AKR1B10; EC 1.1.1.2; Aldo-keto reductase family 1 member B10; ARP; EC 1.1.1.-; ARP; aldo-keto reduct ase family 1, member B10; aldose reductase-like 1; small intestine reductase; aldose reductase-like peptide; aldose reductase-related protein; aldo-keto reductase family 1, member B11 (aldose reductase-like); Aldose reductase-like; SI reductase
• Gene ID: 57016
• mRNA Refseq: NM_020299
• Protein Refseq: NP_064695
• MIM: 604707
• UniProt ID: O60218
• Chromosome Location: 7q33
• Pathway: Ascorbate and aldarate metabolism; Bile acid biosynthesis; Bisphenol A degradation; Butanoate metabolism; Fructose and mannose metabolism; Galactose metabolism; Glycine, serine and threonine metabolism; Linoleic acid metabolism; Lysine degradation; Nucleotide sugars metabolism; Tetrachloroethene degradation
• Function: aldo-keto reductase activity; oxidoreductase activity; protein binding