Recombinant Human AIF1 protein(Met1-Pro147), His-tagged

• Cat.No.: AIF1-8643H
• Product Overview: Recombinant Human AIF1 (P55008-1) (Met1-Pro147) was expressed in E. coli with a polyhistide tag at the N-terminus.

Specification

• Source: E. coli
• Species: Human
• Tag: His
• Protein length: Met1-Pro147
• Form: Lyophilized from sterile PBS, 10% glycerol, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Molecular Mass: The recombinant human AIF1 consists of 162 amino acids and predicts a molecular mass of 18.5 KDa. It migrates as an approximately 18 KDa band in SDS-PAGE under reducing conditions.
• Purity: > 85 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: AIF1 allograft inflammatory factor 1 [ Homo sapiens ]
• Official Symbol: AIF1
• Synonyms: AIF1; allograft inflammatory factor 1; AIF 1; Em:AF129756.17; IBA1; interferon gamma responsive transcript; ionized calcium binding adapter molecule 1; IRT 1; protein G1; ionized calcium-binding adapter molecule 1; allograft inflammatory factor-1 splice variant Hara-1; IRT1; AIF-1; IRT-1
• Gene ID: 199
• mRNA Refseq: NM_001623
• Protein Refseq: NP_001614
• MIM: 601833
• UniProt ID: P55008

Case Study

Case 1: Tian Y, et al. Am J Physiol Cell Physiol. 2009

Endothelial cell activation is crucial for things like blood vessel inflammation, clotting, and new blood vessel formation. A key player in this is a protein called AIF-1. Although we didn't know much about how it works in these cells before, this study aimed to change that. It turns out, AIF-1 shows up in inflamed arteries but not in normal ones, and it's usually low in resting cells unless something triggers it. When they reduced AIF-1 in cells, both cell growth and movement dropped; however, bringing AIF-1 back boosted these activities again. Interestingly, even though cutting down AIF-1 didn't affect the forming of vessel-like structures, having more of it made these structures more complex and plentiful. This suggests AIF-1 plays a big role in how these cells get activated and respond to inflammation.

Fig1. Association of inhibition of AIF-1 expression and EC proliferation.

Fig2. AIF-1 expression modulates microvessel sprouting from aortic rings.

Case 2: Xu T, et al. PLoS One. 2014

The oyster Crassostrea ariakensis, important for China's aquaculture, has faced big challenges due to a nasty infection by rickettsia-like organisms (RLO). Tackling this disease is crucial for oyster farming. Researchers have identified a gene called AIF-1, which plays a role in immune defense and inflammation. In oysters, a version called Ca-AIF1 and its antibody, anti-CaAIF1, were studied. Ca-AIF1 encodes a protein involved in calcium binding and shares similarities with AIF-1 in other animals. It is mostly found in hemocytes, and its expression surges when oysters face RLO/LPS challenges. Using recombinant Ca-AIF1 protein boosts the expression of immune-related genes like LITAF and MyD88, while anti-CaAIF1 reduces inflammation markers and even cuts down cell death caused by the infection.

Fig1. SDS-PAGE analysis of pET-32a-Ca-AIF1 expressed in E.coli BL21 (DE3).

Fig2. Samples were collected at different times after Ca-AIF1 protein (1 μg/ml) incubation. Expression levels were determined by real-time RT-PCR.

Application

AIF1, also known as IBA1, is mostly hanging out in microglia and macrophages in our bodies. It's a big deal when it comes to inflammation and cell growth, and it's tied to a bunch of diseases. When microglia get busy in the brain due to injuries or problems, AIF1 steps up, making it a hot target for potential treatments. Plus, it's found in macrophages dealing with things like atherosclerosis and rheumatoid arthritis, which is why we're looking at it closely in inflammation and autoimmune disorder studies.

Researchers love using recombinant AIF1 protein to dive into its roles in cell pathways and disease mechanics, especially for figuring out microglia activation—key to tackling neuroinflammatory conditions. This protein isn't just sticking to one track; it's also crucial for research into heart and metabolic issues, thanks to its part in vascular and metabolic diseases. In the world of biopharma, recombinant AIF1 is a cornerstone for crafting treatments that manage inflammation, paving the way for new therapeutic breakthroughs.

Fig1. Hypothesis proposed for the role of allograft inflammatory factor-1 in systemic sclerosis pathogenesis. (Francesco Del Galdo, 2006)