Recombinant Human AHSA1, His-tagged
Cat.No. : | AHSA1-499H |
Product Overview : | Recombinant human AHSA1 protein with C-terminal His tag was expressed inE.coli,MW=38 kDa. |
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Species : | Human |
Source : | E.coli |
Tag : | His |
Description : | Activator of 90 kDa heat shock protein ATPase homolog 1 is an enzyme that in humans is encoded by the AHSA1 gene. AHSA1 has been shown to interact with Heat shock protein 90kDa alpha (cytosolic), member A1. The co-chaperone Aha1 (Activator of HSP90 ATPase-1) binds the middle domain of Hsp90(amino acid 272-627) and is proposed to function as an ATPase-activating protein that competes with p23 and other co-chaperones for Hsp90 binding. |
Purity : | >90%. |
Formulation : | 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 30% glycerol, and 3 mM DTT. |
Specific Activity : | The addition of AHA1 increased ATPase activity of hsp90α from 0.18 to 0.94 pmol/min/ug. Assay conditions: 50 μl reaction mix (100 mM Tris-HCl pH7.4, 20 mM KCl, 6 mM MgCl2, 200 Μm ATP, and 5 μg Hsp90α) was incubated with Aha1 (0-10 μg) for 3 hrs at 37ºC. To stop the reaction, 100 μl of the malachite green reagent was added to each well. Absorbanceat 620 nm was measured. |
Application : | ATPase activity assay for Hsp90α. |
Storage : | >6 months at –80°C. |
Gene Name | AHSA1 AHA1, activator of heat shock 90kDa protein ATPase homolog 1 (yeast) [ Homo sapiens ] |
Synonyms | AHSA1; AHA1, activator of heat shock 90kDa protein ATPase homolog 1 (yeast); p38; AHA1; C14orf3; activator of 90 kDa heat shock protein ATPase homolog 1; chromosome 14 open reading frame 3; activator of heat shock 90kDa protein ATPase homolog 1 |
Gene ID | 10598 |
mRNA Refseq | NM_012111 |
Protein Refseq | NP_036243 |
MIM | 608466 |
UniProt ID | O95433 |
Chromosome Location | 14q24 |
Function | ATPase activator activity; ATPase activator activity; chaperone binding; protein binding |
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Not For Human Consumption!
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