Active Recombinant Human ADRBK1 Protein, GST-tagged

• Cat.No.: ADRBK1-383H
• Product Overview: Human ADRBK1 (AAA58391.1, 1 a.a. - 689 a.a.) full-length recombinant protein with GST tag expressed in baculovirus infected Sf21 cells.

Specification

• Species: Human
• Source: Insect Cells
• Tag: GST
• Description: The product of this gene phosphorylates the beta-2-adrenergic receptor and appears to mediate agonist-specific desensitization observed at high agonist concentrations. This protein is an ubiquitous cytosolic enzyme that specifically phosphorylates the activated form of the beta-adrenergic and related G-protein-coupled receptors. Abnormal coupling of beta-adrenergic receptor to G protein is involved in the pathogenesis of the failing heart. [provided by RefSeq, Jul 2008]
• Form: Liquid
• Bio-activity: The activity was determined by ELISA. The enzyme was incubated with biotinylated substrate protein, and after stopping kinase reaction by EDTA, the reaction solution was transferred into streptavidin- coated plate. Phosphorylation was detected by anti-phospho antibody and HRP-labeled anti-rabbit IgG. Substrate: Porcine tublin. ATP: 100 uM.
• Molecular Mass: 106 kDa
• Purity: 55 % by SDS-PAGE/CBB staining
• Applications: Functional Study
  SDS-PAGE
• Storage: Store at -80 centigrade. Aliquot to avoid repeated freezing and thawing.
• Storage Buffer: In 50 mM Tris-HCl, 150 mM NaCl, pH 7.5 (0.1% CHAPS, 1 mM DTT, 10% glycerol)

Gene Information

• Gene Name: ADRBK1 adrenergic, beta, receptor kinase 1 [ Homo sapiens ]
• Official Symbol: ADRBK1
• Synonyms: ADRBK1; adrenergic, beta, receptor kinase 1; beta-adrenergic receptor kinase 1; BARK1; GRK2; beta-ARK-1; G-protein coupled receptor kinase 2; BETA-ARK1; FLJ16718
• Gene ID: 156
• mRNA Refseq: NM_001619
• Protein Refseq: NP_001610
• MIM: 109635
• UniProt ID: P25098