Recombinant Human ACTN2, His-tagged

• Cat.No.: ACTN2-31382TH
• Product Overview: Recombinant fragment, corresponding to amino acids 261-886 of Human Sarcomeric Alpha Actinin, with N terminal His tag, 626 amino acids, 72kDa, ,

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 261-886 a.a.
• Description: Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a muscle-specific, alpha actinin isoform that is expressed in both skeletal and cardiac muscles.
• Conjugation: HIS
• Tissue specificity: Expressed in both skeletal and cardiac muscle.
• Form: Lyophilised:Reconstitution with 136 μl aqua dest.
• Storage buffer: Preservative: NoneConstituents: 0.5% Trehalose, 6M Urea, 100mM Sodium phosphate, 10mM Sodium chloride, pH 4.5
• Storage: Shipped at 4°C. Upon delivery aliquot and store at -80oC. Avoid freeze / thaw cycles.
• Sequences of amino acids: QAETAANRICKVLAVNQENERLMEEYERLASELLEWIRRT IPWLENRTPEKTMQAMQKKLEDFRDYRRKHKPPKVQEK CQLEINFNTLQTKLRISNRPAFMPSEGKMVSDIAGAWQ RLEQAEKGYEEWLLNEIRRLERLEHLAEKFRQKASTHETW AYGKEQILLQKDYESASLTEVRALLRKHEAFESDLAAH QDRVEQIAAIAQELNELDYHDAVNVNDRCQKICDQWDR LGTLTQKRREALERMEKLLETIDQLHLEFAKRAAPFNN WMEGAMEDLQDMFIVHSIEEIQSLITAHEQFKATLPEADG ERQSIMAIQNEVEKVIQSYNIRISSSNPYSTVTMDELR TKWDKVKQLVPIRDQSLQEELARQHANERLRRQFAAQA NAIGPWIQNKMEEIARSSIQITGALEDQMNQLKQYEHNIINYKNNIDKLEGDHQLIQEALVFDNKHTNYTMEHIRVGW ELLLTTIARTINEVETQILTRDAKGITQEQMNEFRASF NHFDRRKNGLMDHEDFRACLISMGYDLGEAEFARIMTL VDPNGQGTVTFQSFIDFMTRETADTDTAEQVIASFRILAS DKPYILAEELRRELPPDQAQYCIKRMPAYSGPGSVPGA LDYAAFSS
• Sequence Similarities: Belongs to the alpha-actinin family.Contains 1 actin-binding domain.Contains 2 CH (calponin-homology) domains.Contains 2 EF-hand domains.Contains 4 spectrin repeats.

Gene Information

• Gene Name: ACTN2 actinin, alpha 2 [ Homo sapiens ]
• Official Symbol: ACTN2
• Synonyms: ACTN2; actinin, alpha 2; alpha-actinin-2
• Gene ID: 88
• mRNA Refseq: NM_001103
• Protein Refseq: NP_001094
• MIM: 102573
• Uniprot ID: P35609
• Chromosome Location: 1q42-q43
• Pathway: Activation of NMDA receptor upon glutamate binding and postsynaptic events, organism-specific biosystem; Adherens junction, organism-specific biosystem; Adherens junction, conserved biosystem; Amoebiasis, organism-specific biosystem; Amoebiasis, conserved biosystem
• Function: FATZ 1 binding; ZASP binding; actin binding; calcium ion binding; identical protein binding