Recombinant Human ABCB1 protein, His & GST-tagged

• Cat.No.: ABCB1-1807H
• Product Overview: Recombinant Human ABCB1 aa. (Phe394~Val672) fused with N-terminal His & GST tag was produced in E. coli cells.

Specification

• Species: Human
• Source: E.coli
• Tag: GST&His
• Protein Length: Phe394~Val672
• Form: Freeze-dried powder
• Molecular Mass: 61kDa as determined by SDS-PAGE reducing conditions.
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Purity: >98%
• Characteristic: The isoelectric point is 7.
• Applications: SDS-PAGE; WB; ELISA; IP; CoIP; ReporterAssays; Purification; Amine Reactive Labeling.
• Stability: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Concentration: 200ug/mL
• Storage buffer: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5%Trehalose and Proclin300.
• Reconstitution: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: ABCB1 ATP binding cassette subfamily B member 1 [ Homo sapiens (human) ]
• Official Symbol: ABCB1
• Synonyms: ABCB1; ATP binding cassette subfamily B member 1; CLCS; MDR1; P-GP; PGY1; ABC20; CD243; GP170; multidrug resistance protein 1; ATP-binding cassette, sub-family B (MDR/TAP), member 1; P glycoprotein; P-glycoprotein 1; colchicin sensitivity; doxorubicin resistance
• Gene ID: 5243
• mRNA Refseq: NM_000927.4
• Protein Refseq: NP_000918.2
• UniProt ID: P08183