Recombinant Human 6-phosphogluconolactonase, His-tagged

• Cat.No.: PGLS-2843H
• Product Overview: Recombinant human PGLS protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. MW = 29.7 kDa (278aa) confirmed by MALDI-TOF.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Description: 6-phosphogluconolactonase, also known PGLS, is an enzyme in the second step pentose phosphate pathway, which is essential for the synthesis of nucleotide sugars and NADPH, the main source of reducing power. It converts 6-phosphogluconolactone to 6-phosphogluconate.
• Sequences Of Amino Acids: MGSSHHHHHH SSGLVPRGSH MAAPAPGLIS VFSSSQELGA ALAQLVAQRA ACCLAGARAR FALGLSGGSL VSMLARELPA AVAPAGPASL ARWTLGFCDE RLVPFDHAES TYGLYRTHLL SRLPIPESQV ITINPELPVE EAAEDYAKKL RQAFQGDSIP VFDLLILGVG PDGHTCSLFP DHPLLQEREK IVAPISDSPK PPPQRVTLTL PVLNAARTVI FVATGEGKAA VLKRILEDQE ENPLPAALVQ PHTGKLCWFL DEAAARLLTV PFEKHSTL
• Purity: > 95% by SDS – PAGE.
• Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol, 100mM NaCl.
• Concentration: 1 mg/ml (determined by Bradford assay).
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.

Gene Information

• Gene Name: PGLS 6-phosphogluconolactonase [ Homo sapiens ]
• Synonyms: PGLS; 6-phosphogluconolactonase; 6PGL; EC 3.1.1.31
• Gene ID: 25796
• mRNA Refseq: NM_012088
• Protein Refseq: NP_036220
• MIM: 604951
• UniProt ID: O95336
• Chromosome Location: 19p13.2
• Pathway: Metabolic pathways; Pentose phosphate pathway
• Function: 6-phosphogluconolactonase activity; hydrolase activity; monosaccharide binding