Recombinant Human 2,4-Dienoyl CoA Reductase 2, Peroxisomal, His-tagged

• Cat.No.: DECR2-1330H
• Product Overview: Recombinant human DECR2 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Description: DECR2, also known as peroxisomal 2,4-dienoyl-CoA reductase, is an auxiliary enzyme of beta-oxidation. DECR2 participates in the degradation of unsaturated fatty enoyl-CoA esters having double bonds in both even- and odd-numbered positions in peroxisome. This protein catalyzes the NADP-dependent reduction of 2,4-dienoyl-CoA to yield trans-3-enoyl-CoA.
• Form: Liquid. In 20 mM Tris-HCl buffer (pH 8.0) containing 1 mM DTT, 40% glycerol, 0.15 M NaCl.
• Molecular Weight: 33.2 kDa (315 aa) confirmed by MALDI-TOF
• Purity: > 95 % by SDS-PAGE
• Concentration: 0.25 mg/ml (determined by Bradford assay)
• Sequences of amino acids: MGSSHHHHHH SSGLVPRGSH MGSMAQPPPD VEGDDCLPAY RHLFCPDLLR DKVAFITGGG SGIGFRIAEI FMRHGCHTVI ASRSLPRVLT AARKLAGATG RRCLPLSMDV RAPPAVMAAV DQALKEFGRI DILINCAAGN FLCPAGALSF NAFKTVMDID TSGTFNVSRV LYEKFFRDHG GVIVNITATL GNRGQALQVH AGSAKAAVDA MTRHLAVEWG PQNIRVNSLA PGPISGTEGL RRLGGPQASL STKVTASPLQ RLGNKTEIAH SVLYLASPLA SYVTGAVLVA DGGAWLTFPN GVKGLPDFAS FSAKL
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
• Pathways: Peroxisome; fatty acid beta-oxidation IV

Gene Information

• Gene Name: DECR2 2,4-dienoyl CoA reductase 2, peroxisomal [ Homo sapiens ]
• Official Symbol: DECR2
• Synonyms: DECR2; 2,4-dienoyl CoA reductase 2, peroxisomal; PDCR; SDR17C1; peroxisomal 2,4-dienoyl-CoA reductase; OTTHUMP00000045513; OTTHUMP00000067571; 2,4-dienoyl-CoA reductase 2; short chain dehydrogenase/reductase family 17C, member 1; EC 1.3.1.34
• Gene ID: 26063
• mRNA Refseq: NM_020664
• Protein Refseq: NP_065715
• UniProt ID: Q9NUI1
• Chromosome Location: 16p13.3
• Function: 2,4-dienoyl-CoA reductase (NADPH) activity; binding; oxidoreductase activity