Recombinant Horse IL2 protein

• Cat.No.: IL2-113E
• Product Overview: Recombinant Horse IL2 (Cys141Ser) protein was expressed in E.coli.

Specification

• Species: Horse
• Source: E.coli
• Tag: Non
• Protein Length: 129
• Description: Produced by T-cells in response to antigenic or mitogenic stimulation, this protein is required for T-cell proliferation and other activities crucial to regulation of the immune response. Can stimulate B-cells, monocytes, lymphokine-activated killer cells, natural killer cells, and glioma cells.
• Form: Lyophilized from a 0.2 µm filtered concentrated solution in PBS, pH 6.5.
• Bio-activity: Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine CTLL-2 cells is less than 1.0 μg/ml, corresponding to a specific activity of > 1000 IU/mg.
• Molecular Mass: Approximately 14.9 kDa, a single non-glycosylated polypeptide chain containing 129 amino acids.
• AA Sequence: APTSSSKRETQQQLKQLQMDLKLLLEGVNNNKNPKLSKMLTFKINMPKKATELKHLQCLEEELKPLEEMLKNFLSKDIKELMSNINVTVLGLKGSETRFTCEYDDETGTIVEFLNKWITFSQSIFSTMT
• Endotoxin: Less than 0.1 EU/µg of rEqIL-2 Cys141Ser as determined by LAL method.
• Purity: >97% by SDS-PAGE and HPLC analyses.
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 centigrade as supplied. 1 month, 2 to 8 centigrade under sterile conditions after reconstitution. 3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
• Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 centigrade. Further dilutions should be made in appropriate buffered solutions.

Gene Information

• Gene Name: IL2
• Official Symbol: IL2
• Synonyms: IL2; interleukin 2; interleukin-2; TCGF; T-cell growth factor; IL-2
• Gene ID: 100034204
• mRNA Refseq: NM_001085433
• Protein Refseq: NP_001078902
• UniProt ID: P37997