Recombinant HCoV-HKU1(isolate N5) Spike Protein, His-tagged

• Cat.No.: Spike-353V
• Product Overview: HCoV-HKU1(isolate N5) S1 protein, His Tag is expressed from human 293 cells (HEK293). It contains AA Ala 13 - Arg 756 (Accession # Q0ZME7-1).

Specification

• Description: It's been reported that Coronavirus can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.
• Source: HEK293
• Species: HCoV-HKU1
• Tag: His
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4, 10% trehalose.
• Molecular Mass: The protein has a calculated MW of 85.7 kDa. The protein migrates as 110-130 kDa under reducing (R) condition (SDS-PAGE) due to Glycosylation.
• Protein length: Ala 13 - Arg 756
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >90% as determined by SDS-PAGE.
• Storage: For long term storage, the product should be stored at lyophilized state at -20°C or lower.
  Please avoid repeated freeze-thaw cycles.
  This product is stable after storage at:
  -20°C to -70°C for 12 months in lyophilized state;
  -70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Synonyms: Spike, S1 protein, Spike glycoprotein Subunit1, S glycoprotein Subunit1, Spike protein S1, COVID-19