Recombinant HCoV-229E N protein(Met1-Asn389), His-tagged

• Cat.No.: N-2413H
• Product Overview: Recombinant Human coronavirus (HCoV-229E) Nucleocapsid (NP_073556.1) (Met1-Asn389) was expressed in E. coli with a polyhistide tag at the N-terminus.

Specification

• Species: HCoV
• Source: E.coli
• Tag: His
• Protein Length: Met1-Asn389
• Form: Lyophilized from sterile 40mM PB, 500mM NaCl, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Molecular Mass: The recombinant heterodimer of Human coronavirus (HCoV-229E) Nucleocapsid consists of 396 amino acids and has a calculated molecular mass of 44.43 KDa.
• Purity: > 90 % as determined by SDS-PAGE.
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.