Recombinant H5N1 (A/bar-headed goose/Qinghai/14/2008) HA Protein, His-tagged

• Cat.No.: HA-288V
• Product Overview: Recombinant extracellular domain of Influenza A virus (A/bar-headed goose/Qinghai/14/2008 (H5N1)) (ACL28277.1) HA (Met 1-Gln 531) with cleavage site mutated (RERRRKKR →TETR,HA1+HA2, uncleaved) was expressed, with a C-terminal polyhistidine tag.

Specification

• Source: Baculovirus-Insect Cells
• Species: H5N1
• Tag: His
• Predicted N Terminal: Asp 17
• Form: Lyophilized from sterile 50mM Tris, 100mM NaCl, pH 8.0, 5%~8% trehalose and mannitol.
• Molecular Mass: The recombinant hemagglutinin of Influenza A virus (A/bar-headed goose/Qinghai/14/2008 (H5N1)) comprises 522 amino acids and has a calculated molecular mass of 59.5 kDa as estimated in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: >85 % as determined by SDS-PAGE.
• Stability: Samples are stable for up to twelve months from date of receipt at -70ºC.
• Storage: Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.
• Protein length: 1-531 a.a.