Recombinant H5N1 (A/Anhui/1/2005) HA protein, His-mFc-tagged

• Cat.No.: HA-16H
• Product Overview: Recombinant H5N1 (A/Anhui/1/2005) HA protein(ABD28180.1)(Met 1-Gln 530) was expressed in HEK293, fused with the C-terminal polyhistidine-tagged Fc region of mouse IgG1 at the C-terminus.

Specification

• Species: H5N1
• Source: HEK293
• Tag: His&mFc
• Protein Length: Met 1-Gln 530
• Tag: C-His-mFc
• Form: Lyophilized from sterile 100mM Glycine, 10mM NaCl, 50mM Tris, pH 7.5.
• Molecular Mass: The secreted recombinant hemagglutinin of Influenza A virus (A/Anhui/1/2005 (H5N1) comprises 754 amino acids with the predicted molecular mass of 85.5 kDa. As a result of glycosylation, it migrates as an approximately 110 kDa band in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 90 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.