Recombinant H4N6 (A/Swine/Ontario/01911-1/99) HA Protein, His-tagged

• Cat.No.: HA-376V
• Product Overview: Recombinant extracellular domain (Met 1-Ile 528) of the influenza A hemagglutinin (A/Swine/Ontario/01911-1/99 (H4N6)) (AAG17429.1) (HA1+HA2, uncleaved) was expressed, fused with a C-terminal polyhistidine tag.

Specification

• Species: H4N6
• Source: HEK293
• Tag: His
• Protein Length: 1-528 a.a.
• Predicted N Terminal: Gln 17
• Form: Lyophilized from sterile PBS, pH 7.4, 5%~8% trehalose and mannitol.
• Molecular Mass: The secreted recombinant influenza A H4N6 HA (A/Swine/Ontario/01911-1/99 (H4N6)) comprises 523 amino acids and has a predicted molecular mass of 59 kDa. As a result of glycosylation, it migrates as an approximately 65-75 kDa band in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: >92 % as determined by SDS-PAGE.
• Stability: Samples are stable for up to twelve months from date of receipt at -70ºC.
• Storage: Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.