Recombinant H1N1 (A/Puerto Rico/8/1934) HA (ΔTM) Protein, His-tagged

• Cat.No.: HA-1869H
• Product Overview: Recombinant H1N1 (A/Puerto Rico/8/1934) HA (ΔTM) ProteinABD77675.1) (Met1-Gln528) was expressed in HEK293, fused with a C-terminal polyhistidine tag.

Specification

• Species: H1N1
• Source: HEK293
• Tag: His
• Protein Length: Met1-Gln528
• Form: Lyophilized from sterile 20mM Tris, 150mM NaCl, 0.1M Arginase, 0.1M glutamic acid, 10% Glycine, 0.01% tween20, pH8.5
• Molecular Mass: The secreted recombinant influenza A H1N1 (A/Puerto Rico/8/1934 (H1N1)) HA comprises 522 amino acids and has a predicted molecular mass of 59 kDa. As a result of glycosylation, it migrates as an approximately 60-70 kDa band in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 85 % as determined by SDS-PAGE.
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
  Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.