Recombinant Full Length Mouse Uba5 Protein, Myc/DDK-tagged
Cat.No. : | Uba5-6767M |
Product Overview : | Purified recombinant protein of mouse full-length ubiquitin-like modifier activating enzyme 5 (Uba5), with C-terminal MYC/DDK tag, expressed in HEK293T cells. |
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Description : | E1-like enzyme which specifically catalyzes the first step in ufmylation. Activates UFM1 by first adenylating its C-terminal glycine residue with ATP, and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding a UFM1-E1 thioester and free AMP. Activates UFM1 via a trans-binding mechanism, in which UFM1 interacts with distinct sites in both subunits of the UBA5 homodimer. Trans-binding also promotes stabilization of the UBA5 homodimer, and enhances ATP-binding. Transfer of UFM1 from UBA5 to the E2-like enzyme UFC1 also takes place using a trans mechanism. Ufmylation is involved in reticulophagy (also called ER-phagy) induced in response to endoplasmic reticulum stress. Ufmylation is essential for erythroid differentiation of both megakaryocytes and erythrocytes. |
Source : | HEK293T |
Species : | Mouse |
Tag : | Myc&DDK |
Molecular Mass : | 44.8 kDa |
Purity : | > 80% as determined by SDS-PAGE and Coomassie blue staining |
Stability : | Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles. |
Storage : | Store at -80 centigrade after receiving vials. |
Concentration : | >50 μg/mL as determined by microplate BCA method |
Storage Buffer : | 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol. |
Gene Name | Uba5 ubiquitin-like modifier activating enzyme 5 [ Mus musculus (house mouse) ] |
Official Symbol | Uba5 |
Synonyms | UBA5; ubiquitin-like modifier activating enzyme 5; ubiquitin-like modifier-activating enzyme 5; UFM1-activating enzyme; ubiquitin-activating enzyme 5; ubiquitin-activating enzyme E1-domain containing 1; ubiquitin-activating enzyme E1 domain-containing protein 1; Ube1dc1; AW240750; 5730525G14Rik |
Gene ID | 66663 |
mRNA Refseq | NM_025692 |
Protein Refseq | NP_079968 |
UniProt ID | Q8VE47 |
Not For Human Consumption!
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