Recombinant Full Length Mouse Postn, Isoform 2, His tagged

• Cat.No.: Postn-3297M
• Product Overview: Recombinant Mouse POSTN (NP_056599.1) (Met 1-Gln 811) was expressed in HEK293, with a polyhistidine tag at the C-terminus.

Specification

• Source: HEK293
• Species: Mouse
• Tag: C-His
• Protein length: 1-811 a.a.
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured by its ability to induce adhesion of ATDC5 mouse chondrogenic cells.When cells are added to POSTN-His coated plates (10 μg/mL, 100 μL/well), approximately >40% will adhere specifically after 30 minutes at 37°C.
• Molecular Mass: The secreted recombinant mouse POSTN consists of 799 amino acids and has a calculated molecular mass of 89 kDa. The apparent molecular mass of rmPOSTN is approximately 80-85 kDa in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: > 92 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Postn periostin, osteoblast specific factor [ Mus musculus ]
• Official Symbol: Postn
• Synonyms: POSTN; periostin, osteoblast specific factor; periostin; periostin-like factor; osteoblast-specific factor 2; osteoblast specific factor 2 (fasciclin I-like); PN; PLF; Osf2; peri; OSF-2; AI747096; A630052E07Rik
• Gene ID: 50706
• mRNA Refseq: NM_001198765
• Protein Refseq: NP_001185694