Recombinant Full Length Human phosphoglycerate dehydrogenase Protein, His&Flag&StrepII tagged

• Cat.No.: PHGDH-107H
• Product Overview: Recombinant human PHGDH protein (O43175) (Met 1-Phe 533) with a Twin strep tag at the N-terminus and both His, Flag tag at the C-terminus was expressed in E. coli.

Specification

• Species: Human
• Source: E.coli
• Tag: Flag&His&Twin Strep
• Protein Length: 1-533aa
• Description: This gene encodes the enzyme which is involved in the early steps of L-serine synthesis in animal cells. L-serine is required for D-serine and other amino acid synthesis. The enzyme requires NAD/NADH as a cofactor and forms homotetramers for activity. Mutations in this gene have been found in a family with congenital microcephaly, psychomotor retardation and other symptoms. Multiple alternatively spliced transcript variants have been found, however the full-length nature of most are not known. Catalyzes the reversible oxidation of 3-phospho-D-glycerate to 3-phosphonooxypyruvate, the first step of the phosphorylated L-serine biosynthesis pathway. Also catalyzes the reversible oxidation of 2-hydroxyglutarate to 2-oxoglutarate and the reversible oxidation of (S)-malate to oxaloacetate.
• Tag: N-Twin strep and C-His&Flag
• Molecular Mass: 63.3 kDa
• Endotoxin: Less than 1.0 EU/μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Storage: Please avoid repeated freeze-thaw cycles. Samples are stable for up to twelve months from date of receipt at -20 to -80 centigrade. It is recommended that aliquot the reconstituted solution to minimize freeze-thaw cycles.
• Storage Buffer: Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4, 5% Trehalose, 5% mannitol.
• Reconstitution: Reconstitute at 250 μg/mL in sterile water.

Gene Information

• Gene Name: PHGDH phosphoglycerate dehydrogenase [ Homo sapiens (human) ]
• Official Symbol: PHGDH
• Synonyms: PHGDH; phosphoglycerate dehydrogenase; D-3-phosphoglycerate dehydrogenase; PDG; PGDH; SERA; 3-phosphoglycerate dehydrogenase; PGD; PGAD; 3PGDH; 3-PGDH; MGC3017
• Gene ID: 26227
• mRNA Refseq: NM_006623
• Protein Refseq: NP_006614
• MIM: 606879
• UniProt ID: O43175