Recombinant Full Length Human NEDD8 Protein, Rhodamine 110 Labeled

• Cat.No.: NEDD8-02HFL
• Product Overview: This product consists of a full-length, mature NEDD8 polypeptide recombinantly expressed in E. coli, conjugated on its c-terminus to a quenched Rhodamine 110 dye. Once hydrolyzed the free rhodamine provides excellent utility for real time assessment of enzyme activity at excitation (485 nm) and emission (535 nm).

Specification

• Description: NEDD8 (Neural Precursor Cell Expressed, Developmentally Down-Regulated 8) is a ubiquitin like protein that plays an important role in regulating development and the cell cycle. NEDD8 is conjugated to a target protein via a signaling cascade similar to ubiquitin: a NEDD8 specific E1 activating enzyme (APPBP1/UBA3) adenylates the c-terimnus of NEDD8 which is than subsequently passed to an E2 conjugating enzyme (UBE2M or UBE2F) that facilitates covalent attachment of NEDD8 to a cullin subunit of an SCF E3 ubiquitin ligase.
• Source: E. coli
• Species: Human
• Protein length: 1-81aa
• Conjugation/Label: Rhodamine 110
• Molecular Mass: 8.9 kDa
• Purity: >97% by LCMS
• Usage: Typical working range: 50-500nM
• Applications: NEDD8-Rhodamine110 can be used as a substrate for enzymes exhibiting deNEDDylating activity, e.g UCH-L3, UCH-L1, COP9 Signalosome, and NEDP1.
• Notes: For Research Use Only, Not For Use In Humans.
• Storage: Store at -80 centigrade. Avoid multiple freeze/thaws.
• Storage Buffer: 50mM MES pH 6.0, 150mM NaCl
• Concentration: 170 μM, 1.5 mg/mL
• Detection Method: Fluorescence
• Substrate Properties: Protein-Based Substrate
• Excitation/Emission (nm): The Excitation and Emission of this substrate is 485nm and 535nm respectively.

Gene Information

• Gene Name: NEDD8 neural precursor cell expressed, developmentally down-regulated 8 [ Homo sapiens ]
• Official Symbol: NEDD8
• Synonyms: NEDD8; neural precursor cell expressed, developmentally down-regulated 8; Nedd 8; neddylin; ubiquitin-like protein Nedd8; NEDD-8; FLJ43224; MGC104393; MGC125896; MGC125897
• Gene ID: 4738
• mRNA Refseq: NM_006156
• Protein Refseq: NP_006147
• MIM: 603171
• UniProt ID: Q15843