Recombinant Full Length Human NEDD8 Protein, AMC Labeled
| Cat.No. : | NEDD8-03HFL |
| Product Overview : | This product consists of a full-length, mature NEDD8 polypeptide (amino acids 1-76) recombinantly expressed in E. coli, conjugated on its c-terminus to a quenched AMC110 dye. Once hydrolyzed the free AMC provides excellent utility for real time assessment of enzyme activity at excitation (345 nm) and emission (445 nm). Typical working range is 50-500nM. |
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| Description : | NEDD8 (Neural Precursor Cell Expressed, Developmentally Down-Regulated 8) is a ubiquitin like protein that plays an important role in regulating development and the cell cycle. NEDD8 is conjugated to a target protein via a signaling cascade similar to ubiquitin: a NEDD8 specific E1 activating enzyme (APPBP1/UBA3) adenylates the c-terimnus of NEDD8 which is than subsequently passed to an E2 conjugating enzyme (UBE2M or UBE2F) that facilitates covalent attachment of NEDD8 to a cullin subunit of an SCF E3 ubiquitin ligase. |
| Source : | E. coli |
| Species : | Human |
| Protein length : | 1-81aa |
| Conjugation/Label : | AMC |
| Molecular Mass : | 8.7 kDa |
| Purity : | >99% by LCMS |
| Usage : | Typical working range: 50-500nM |
| Applications : | NEDD8-Rh 110 can be used as a substrate for enzymes exhibiting deNEDDylating activity, e.g UCH-L3, UCH-L1, COP9 Signalosome, and NEDP1. |
| Notes : | For Research Use Only, Not For Use In Humans. |
| Storage : | Store at -80 centigrade. Avoid multiple freeze thaw cycles. |
| Storage Buffer : | 50mM MES, pH 6.0 |
| Concentration : | 200 μM, 1.74 mg/mL |
| Detection Method : | Fluorescence |
| Substrate Properties : | Protein-Based Substrate |
| Excitation/Emission (nm) : | The Excitation and Emission of this substrate is 345nm and 445nm respectively. |
| Gene Name | NEDD8 neural precursor cell expressed, developmentally down-regulated 8 [ Homo sapiens ] |
| Official Symbol | NEDD8 |
| Synonyms | NEDD8; neural precursor cell expressed, developmentally down-regulated 8; Nedd 8; neddylin; ubiquitin-like protein Nedd8; NEDD-8; FLJ43224; MGC104393; MGC125896; MGC125897; |
| Gene ID | 4738 |
| mRNA Refseq | NM_006156 |
| Protein Refseq | NP_006147 |
| MIM | 603171 |
| UniProt ID | Q15843 |
Not For Human Consumption!
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