Recombinant Full Length Human FLI1 Protein, C-Flag-tagged

• Cat.No.: FLI1-286HFL
• Product Overview: Recombinant Full Length Human FLI1 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: This gene encodes a transcription factor containing an ETS DNA-binding domain. The gene can undergo a t(11;22)(q24;q12) translocation with the Ewing sarcoma gene on chromosome 22, which results in a fusion gene that is present in the majority of Ewing sarcoma cases. An acute lymphoblastic leukemia-associated t(4;11)(q21;q23) translocation involving this gene has also been identified. Alternative splicing results in multiple transcript variants.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 50.8 kDa
• AA Sequence: MDGTIKEALSVVSDDQSLFDSAYGA AAHLPKADMTASGSPDYGQPHKINP LPPQQEWINQPVRVNVKREY DHMNGSRESPVDCSVSKCSKLVGGG ESNPMNYNSYMDEKNGPPPPNMTTN ERRVIVPADPTLWTQEHVRQ WLEWAIKEYSLMEIDTSFFQNMDGK ELCKMNKEDFLRATTLYNTEVLLSH LSYLRESSLLAYNTTSHTDQ SSRLSVKEDPSYDSVRRGAWGNNMN SGLNKSPPLGGAQTISKNTEQRPQP DPYQILGPTSSRLANPGSGQ IQLWQFLLELLSDSANASCITWEGT NGEFKMTDPDEVARRWGERKSKPNM NYDKLSRALRYYYDKNIMTK VHGKRYAYKFDFHGIAQALQPHPTE SSMYKYPSDISYMPSYHAHQQKVNF VPPHPSSMPVTSSSFFGAAS
  QYWTSPTGGIYPNPNVPRHPNTHVP SHLGSYYTRTRPLEQKLISEEDLAA NDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Transcription Factors

Gene Information

• Gene Name: FLI1 Fli-1 proto-oncogene, ETS transcription factor [ Homo sapiens (human) ]
• Official Symbol: FLI1
• Synonyms: EWSR2; FLI-1; SIC-1; BDPLT21
• Gene ID: 2313
• mRNA Refseq: NM_002017.5
• Protein Refseq: NP_002008.2
• MIM: 193067
• UniProt ID: Q01543