Recombinant Full Length Human CYP8B1 Protein, GST-tagged

• Cat.No.: CYP8B1-2485HF
• Product Overview: Human CYP8B1 full-length ORF ( NP_004382.1, 1 a.a. - 501 a.a.) recombinant protein with GST-tag at N-terminal.

Specification

• Description: This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This endoplasmic reticulum membrane protein catalyzes the conversion of 7 alpha-hydroxy-4-cholesten-3-one into 7-alpha,12-alpha-dihydroxy-4-cholesten-3-one. The balance between these two steroids determines the relative amounts of cholic acid and chenodeoxycholic acid both of which are secreted in the bile and affect the solubility of cholesterol. This gene is unique among the cytochrome P450 genes in that it is intronless. [provided by RefSeq, Jul 2008]
• Source: In Vitro Cell Free System
• Species: Human
• Tag: GST
• Molecular Mass: 84.5 kDa
• Protein length: 501 amino acids
• AA Sequence: MVLWGPVLGALLVVIAGYLCLPGMLRQRRPWEPPLDKGTVPWLGHAMAFRKNMFEFLKRMRTKHGDVFTVQLGGQYFTFVMDPLSFGPILKDTQRKLDFGQYAKKLVLKVFGYRSVQGDHEMIHSASTKHLRGDGLKDLNETMLDSLSFVMLTSKGWSLDASCWHEDSLFRFCYYILFTAGYLSLFGYTKDKEQDLLQAGELFMEFRKFDLLFPRFVYSLLWPREWLEVGRLQRLFHKMLSVSHSQEKEGISNWLGNMLQFLREQGVPSAMQDKFNFMMLWASQGNTGPTSFWALLYLLKHPEAIRAVREEATQVLGEARLETKQSFAFKLGALQHTPVLDSVVEETLRLRAAPTLLRLVHEDYTLKMSSGQEYLFRHGDILALFPYLSVHMDPDIHPEPTVFKYDRFLNPNGSRKVDFFKTGKKIHHYTMPWGSGVSICPGRFFALSEVKLFILLMVTHFDLELVDPDTPLPHVDPQRWGFGTMQPSHDVRFRYRLHPTE
• Applications: Enzyme-linked Immunoabsorbent Assay
  Western Blot (Recombinant protein)
  Antibody Production
  Protein Array
• Notes: Best use within three months from the date of receipt of this protein.
• Storage: Store at -80 centigrade. Aliquot to avoid repeated freezing and thawing.
• Storage Buffer: 50 mM Tris-HCl, 10 mM reduced Glutathione, pH=8.0 in the elution buffer.

Gene Information

• Gene Name: CYP8B1 cytochrome P450 family 8 subfamily B member 1 [ Homo sapiens (human) ]
• Official Symbol: CYP8B1
• Synonyms: CYP8B1; cytochrome P450 family 8 subfamily B member 1; Cytochrome P450 Family 8 Subfamily B Member 1; Cytochrome P450, Subfamily VIIIB (Sterol 12-Alpha-Hydroxylase), Polypeptide 1; Cytochrome P450, Family 8, Subfamily B, Polypeptide 1; Sterol 12-Alpha-Hydroxylase; Cytochrome P450 8B1; CYPVIIIB1; CYP12; 7 Alpha-Hydroxy-4-Cholesten-3-One 12-Alpha-Hydroxylase; 7-Alpha-Hydroxycholest-4-En-3-One 12-Alpha-Hydroxylase; 7-Alpha-Hydroxy-4-Cholesten-3-One 12-Alpha-Hydroxylase; EC 1.14.18.8; CP8B; 7-alpha-hydroxycholest-4-en-3-one 12-alpha-hydroxylase; 7 alpha-hydroxy-4-cholesten-3-one 12-alpha-hydroxylase; CYPVIIIB1; cytochrome P450 8B1; cytochrome P450, family 8, subfamily B, polypeptide 1; cytochrome P450, subfamily VIIIB; (sterol 12-alpha-hydroxylase), polypeptide 1; sterol 12-alpha-hydroxylase; EC 1.14.18.8
• Gene ID: 1582
• mRNA Refseq: NM_004391
• Protein Refseq: NP_004382
• MIM: 602172
• UniProt ID: Q9UNU6