Recombinant Full Length Human CRBN Protein, N-Flag-tagged

• Cat.No.: CRBN-13HFL
• Product Overview: Recombinant Full Length Human CRBN Protein, fused to Flag-tag at N-terminus, was expressed in HEK293T.

Specification

• Species: Human
• Source: HEK293
• Tag: Flag
• Protein Length: 2-422 aa(end)
• Description: The Cereblon (CRBN) protein via its interaction with the DNA damage-binding protein-1 (DDB1), Cullin 4 (Cul4A or Cul4B), and regulator of Cullins 1 (RoC1) forms a functional E3 Ub ligase complex. In this complex, Cereblon functions as a substrate receptor that mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Like most E3 ligases, Cereblon complex ubiquitinates itself and its auto-ubiquitination promotes its ligase activity. Cereblon complex is involved in many biological processes including cell proliferation and apoptosis, and it has been extensively used in targeted protein degradation (PROTAC®), where CRBN binds to the protein of interest and leads to its degradation. This approach has shown promising results in the treatment of cancer.
• Form: Aqueous buffer solution. 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 20% glycerol, and 100 μg ml FLAG peptide.
• Molecular Mass: 51 kDa
• Purity: ≥70%
• Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
• Storage: -80 centigrade
• Concentration: 0.31 mg/ml
• Use/Stability: At least 6 months at -80 centigrade. Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Gene Information

• Gene Name: CRBN cereblon [ Homo sapiens (human) ]
• Official Symbol: CRBN
• Synonyms: CRBN; cereblon; AD-006; MRT2; MRT2A
• Gene ID: 51185
• mRNA Refseq: NM_016302.4
• Protein Refseq: NP_057386.2
• MIM: 609262
• UniProt ID: Q96SW2