Recombinant Full Length Human CDK2 Protein, C-Flag-tagged

• Cat.No.: CDK2-472HFL
• Product Overview: Recombinant Full Length Human CDK2 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: This gene encodes a member of a family of serine/threonine protein kinases that participate in cell cycle regulation. The encoded protein is the catalytic subunit of the cyclin-dependent protein kinase complex, which regulates progression through the cell cycle. Activity of this protein is especially critical during the G1 to S phase transition. This protein associates with and regulated by other subunits of the complex including cyclin A or E, CDK inhibitor p21Cip1 (CDKN1A), and p27Kip1 (CDKN1B). Alternative splicing results in multiple transcript variants.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 33.7 kDa
• AA Sequence: MENFQKVEKIGEGTYGVVYKARNKL TGEVVALKKIRLDTETEGVPSTAIR EISLLKELNHPNIVKLLDVI HTENKLYLVFEFLHQDLKKFMDASA LTGIPLPLIKSYLFQLLQGLAFCHS HRVLHRDLKPQNLLINTEGA IKLADFGLARAFGVPVRTYTHEVVT LWYRAPEILLGCKYYSTAVDIWSLG CIFAEMVTRRALFPGDSEID QLFRIFRTLGTPDEVVWPGVTSMPD YKPSFPKWARQDFSKVVPPLDEDGR SLLSQMLHYDPNKRISAKAA
  LAHPFFQDVTKPVPHLRLTRTRPLE QKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Cell cycle, Oocyte meiosis, p53 signaling pathway, Pathways in cancer, Progesterone-mediated oocyte maturation, Prostate cancer, Small cell lung cancer

Gene Information

• Gene Name: CDK2 cyclin dependent kinase 2 [ Homo sapiens (human) ]
• Official Symbol: CDK2
• Synonyms: CDKN2; p33(CDK2)
• Gene ID: 1017
• mRNA Refseq: NM_001798.5
• Protein Refseq: NP_001789.2
• MIM: 116953
• UniProt ID: P24941

Reviews

04/14/2022

Product met expectations, happy.

03/22/2022

High quality, reliable results.

05/31/2021

Outstanding performance, highly recommend.

Q&As

What is the primary role of CDK2 in cell cycle regulation?

CDK2 is crucial for driving cells through the G1/S and S phases of the cell cycle.

What role does CDK2 play in cellular response to DNA damage?

CDK2 helps in cell cycle arrest to allow DNA repair, preventing mutation accumulation.

What are the key regulatory mechanisms controlling CDK2 activity?

CDK2 activity is controlled by cyclins, CDK inhibitors, and phosphorylation status.

How is dysregulation of CDK2 implicated in cancer development?

CDK2 dysregulation can lead to uncontrolled cell proliferation, contributing to cancer.

Are there existing or potential therapeutic strategies targeting CDK2?

Targeting CDK2 with specific inhibitors is explored as a potential cancer therapy strategy.

How is CDK2 activity linked to cell cycle checkpoints?

It is integral to cell cycle checkpoints ensuring DNA integrity before replication.

How does CDK2 contribute to DNA replication?

It phosphorylates proteins necessary for DNA replication initiation.