Recombinant Full Length Human CCT4 Protein, Flag-tagged

• Cat.No.: CCT4-10HFL
• Product Overview: Recombinant protein of human chaperonin containing TCP1, subunit 4 (delta) (CCT4) with C-Flag tag was expressed in HEK293.

Specification

• Description: The chaperonin containing TCP1 complex (CCT), also called the TCP1 ring complex, consists of 2 back-to-back rings, each containing 8 unique but homologous subunits, such as CCT4. CCT assists the folding of newly translated polypeptide substrates through multiple rounds of ATP-driven release and rebinding of partially folded intermediate forms. Substrates of CCT include the cytoskeletal proteins actin and tubulin, as well as alpha-transducin.
• Source: HEK293
• Species: Human
• Tag: C-Flag
• Protein length: 1-539 aa
• Molecular Mass: 57.7 kDa
• AA Sequence: MPENVAPRSGATAGAAGGRGKGAYQDRDKPAQIRFSNISAAKAVADAIRTSLGPKGMDKMIQDGKGDVTITNDGATILKQMQVLHPAARMLVELSKAQDIEAGDGTTSVVIIAGSLLDSCTKLLQKGIHPTIISESFQKALEKGIEILTDMSRPVELSDRETLLNSATTSLNSKVVSQYSSLLSPMSVNAVMKVIDPATATSVDLRDIKIVKKLGGTIDDCELVEGLVLTQKVSNSGITRVEKAKIGLIQFCLSAPKTDMDNQIVVSDYAQMDRVLREERAYILNLVKQIKKTGCNVLLIQKSILRDALSDLALHFLNKMKIMVIKDIEREDIEFICKTIGTKPVAHIDQFTADMLGSAELAEEVNLNGSGKLLKITGCASPGKTVTIVVRGSNKLVIEEAERSIHDALCVIRCLVKKRALIAGGGAPEIELALRLTEYSRTLSGMESYCVRAFADAMEVIPSTLAENAGLNPISTVTELRNRHAQGEKTAGINVRKGGISNILEELVVQPLLVSVSALTLATETVRSILKIDDVVNTRTRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Notes: For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Storage Buffer: 25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol
• Concentration: > 0.05 μg/μL as determined by microplate BCA method

Gene Information

• Gene Name: CCT4 chaperonin containing TCP1 subunit 4 [ Homo sapiens (human) ]
• Official Symbol: CCT4
• Synonyms: CCT4; chaperonin containing TCP1 subunit 4; SRB; Cctd; CCT-DELTA; T-complex protein 1 subunit delta; TCP-1-delta; chaperonin containing T-complex polypeptide 1 subunit 4; chaperonin containing TCP1, subunit 4 (delta); chaperonin containing t-complex polypeptide 1, delta subunit; stimulator of TAR RNA-binding
• Gene ID: 10575
• mRNA Refseq: NM_006430
• Protein Refseq: NP_006421
• MIM: 605142
• UniProt ID: P50991