Recombinant Full Length E3 Ubiquitin-Protein Ligase Hrd-1(Sel-11) Protein, His-Tagged

• Cat.No.: RFL20820CF
• Product Overview: Recombinant Full Length E3 ubiquitin-protein ligase hrd-1(sel-11) Protein (A8Y4B2) (24-622aa), fused to N-terminal His tag, was expressed in E. coli.

Specification

• Species: Caenorhabditis briggsae
• Source: E.coli
• Tag: His
• ProteinLength: Full Length of Mature Protein (24-622)
• Form: Lyophilized powder
• AA Sequence: FVINKQFYPSIVYLSKSNASMAVLYFQGIVLVYLMFQLLKSILFGDLRAAEAEHLSERTW HAVLETCLAFTVFRDDFSAMFVMQFIGLLFIKCFHWLADDRVDMMERSPVITLRFHLRMM TVLAALGFADSYFVSSAYFSTITKGASSQIVFGFEYAILLALVLHVTIKYLLHMHDLRNP QSWDNKAVYLLYAELLINLIRCVLYGFFAVIMLRVHTFPLFSVRPFYQSVRALHKAFLDV ILSRRAINAMNSQFPVVSNDELSAMDATCIICREEMTVESSPKRLPCSHVFHAHCLRSWF QRQQTCPTCRTDIWQGRNGAAGGANAGGAAENNAAGAPPAAGIPPFLPFLGHQFGFPQAA AGAQVGGAQAGGHPGPFPHQIFYAPAPANRPEFMNLAPPPMPMAGPPGMFPMMPPPPIPQ PNAAPGESSNAEPPGRPNFDRFSVEELHRMEGDMRDAILARIQAMENIMVILESAQVQMV QLAAITPLRRPVPTAEASEEETATASSVPTSVPSEEPSPAPSTPETASAPRSMFRGNLFN DTQSTSTPSTSAGPQPSLTPSTSSVPSTSSVRTPEADEVRQRRLAHLNARFPPPNPEHE
• Purity: Greater than 90% as determined by SDS-PAGE.
• Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
• Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
• Storage Buffer: Tris/PBS-based buffer, 6% Trehalose, pH 8.0
• Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Gene Information

• Gene Name: sel-11
• Synonyms: sel-11; hrd-1; CBG23271; E3 ubiquitin-protein ligase hrd-1; RING-type E3 ubiquitin transferase hrd-1; Suppressor/enhancer of lin-12
• UniProt ID: A8Y4B2