Recombinant FIV gp40 Envelope protein, His&TrxA tagged

• Cat.No.: env-014F
• Product Overview: gp40 is the transmembrane part of the envelope glycoprotein gp160 (aa 634-770) which has been expressed as a recombinant antigen fused to the thioredoxin TrxA and a his-tag in its N-terminus.

Specification

• Species: FIV
• Source: E.coli
• Tag: His&TRxA
• Description: gp40 -is a transmembrane glycoprotein responsible for viral membrane fusion with the host cell membrane. The gp40 subunit contains different functional regions in its ectodomain: the fusion peptide (FP), heptad repeat 1 (HR1), HR2, and the transmembrane region. This protein is very similar to gp41 from HIV. The sequence of gp40 contains four functional regions, as follows: the N-terminal fusion peptide (which is thought to insert into the host cell membrane) is followed by an ectodomain, a transmembrane region and a cytoplasmic domain.
• Tag: N-His&TrxA
• Molecular Mass: Determined by SDS-PAGE, the protein bands are two and between molecular markers of 35-45 kDa, while relative molecular mass calculated from amino acid sequence is 38.276,75 Da.
• Purity: > 95% by observation on SDS-PAGE electrophoresis
• Applications: WB, DB, IE, DE
• Storage: Upon arrival, it should be aliquoted to avoid repeated freezing and thawing cycles and stored at -20 to -80 centigrade. In order to defrost the protein, maintain the aliquot at 25 centigrade without shaking to avoid aggregation.
• Storage Buffer: 20 mM HEPES pH 8, 0.15 M NaCl, 0.13 M trehalose, 0.5 M arginine and 0.1% polyoxyethylene (10) tridecyl ether
• Concentration: 1.11 mg/mL
• Shipping: Protein is shipped with dry ice.
• Reference: 1. Gill SC, von Hippel PH. Calculation of protein extinction coefficients from amino acid sequence data. Anal Biochem. 1989 Nov 1;182(2):319-26.