Recombinant EV71 G protein(Met1-Gln323), His&GST-tagged

• Cat.No.: G-2377E
• Product Overview: Recombinant Human enterovirus 71 genome polyprotein (Q66478-1)(Met 1-Gln 323), corresponding to the protein VP0 precusor, a component of immature procapsids, was expressed in Insect Cells, fused with the N-terminal polyhistidine tag followed by a GST tag

Specification

• Species: EV71
• Source: Insect Cells
• Tag: GST&His
• Protein Length: Met1-Gln323
• Form: Lyophilized from sterile 50mM Tris, 100mM NaCl, 2mM GSH, 0.5mM PMSF, pH 8.0. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Molecular Mass: The recombinant human enterovirus 71 protein VP0/GST chimera consists of 560 amino acids and has a calculated molecular mass of 63 kDa. It migrates as an approximately 58 kDa band in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 85 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.