Recombinant EV-A71 VP1 Protein (Gly1-Ser297), C-His tagged, Animal-free, Carrier-free

• Cat.No.: VP1-327E
• Product Overview: Recombinant Enterovirus A71 VP1 Protein (Gly1-Ser297) with C-His tag was expressed in E. coli and Animal-free as well as Carrier-free.

Specification

• Species: EV-A71
• Source: E.coli
• Tag: His
• Protein Length: Gly1-Ser297
• Description: Enterovirus A71 (EV-A71), a positive-strand RNA virus of the Picornaviridae family, is a persisting global concern. EV-A71 is primarily transmitted through the fecal-oral route, causing hand, foot, and mouth disease (HFMD), severe central nervous system complications, neural damage, and even life-threatening. VP1 is a structural capsid protein consisting of 297 amino acids, promoting infection by binding to specific receptors on the surface of host cells, including P-selectin glycoprotein ligand 1 (PSGL-1), scavenger receptor B2 (SCARB2), annexin II protein, sialylated glycan, and heparan sulfate.
• Form: Lyophilized
• Molecular Mass: The protein has a calculated MW of 33.76 kDa.
  The protein migrates as 35 kDa under reducing condition (SDS-PAGE analysis).
• Purity: >95% as determined by SDS-PAGE analysis.
• Storage: This product is stable after storage at:
  -20 centigrade for 12 months in lyophilized state from date of receipt.
  -20 or -80 centigrade for 1 month under sterile conditions after reconstitution.
  Avoid repeated freeze/thaw cycles.
• Storage Buffer: Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
• Reconstitution: It is recommended to reconstitute the lyophilized protein in sterile H2O to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.
• Shipping: The product is shipped with polar packs. Upon receipt, store it immediately at -20 centigrade or lower for long term storage.

Gene Information

• Official Symbol: VP1
• Synonyms: VP1