Recombinant Enterobacteria phage phiX174 RepA Protein (Full Length), N-His tagged

• Cat.No.: RepA-1638E
• Product Overview: Recombinant Enterobacteria phage phiX174 A (full length) fused with the N-His tag was expressed in E. coli.

Specification

• Species: Enterobacteria phage phiX174
• Source: E.coli
• Tag: His
• Protein Length: Full Length
• Description: Plays an essential role in viral DNA replication. Binds the origin of replication and cleaves the dsDNA replicative form I (RFI) and becomes covalently bound to it via phosphotyrosine bond, generating the dsDNA replicative form II (RFII). In turn, viral DNA replication initiates in the presence of host Rep and DNA polymerase III at the 3'-OH of the cleavage site. After one round of rolling circle synthesis, protein A is linked to the newly synthesized ssDNA and joins the ends of the displaced strand to generate a circular single-stranded molecule ready to be packed into a virion.
• Form: Lyophilized powder/frozen liquid
• Molecular Mass: 78.31 kDa
• Purity: >90% as determined by SDS-PAGE.
• Notes: For research use only.
• Storage: Use a manual defrost freezer and avoid repeated freeze thaw cycles.
  Store at 2 to 8 centigrade for one week.
  Store at -20 to -80 centigrade for twelve months from the date of receipt.
• Storage Buffer: Supplied as solution form in PBS, pH 7.5, 0.02% sarcosyl or lyophilized from PBS, pH 7.5, 0.02% sarcosyl.
• Reconstitution: Reconstitute in sterile water for a stock solution.
• Shipping: They are shipped out with dry ice/blue ice unless customers require otherwise.

Gene Information

• Official Symbol: RepA
• Synonyms: Replication-associated protein A; A; EC:3.1.21.-; EC:6.5.1.1; RepA
• UniProt ID: P03631