Recombinant Ebola Zaire Nucleoprotein

• Cat.No.: NP-89E
• Product Overview: Nucleoprotein (NP) of Ebola virus has strong antigenicity in immune reactions. C-terminal of EBO-Z nucleoprotein was expressed and purified from E. coli, it migrated at 15kDa on SDS-PAGE and pI is 4.87. Ebola Zaire was purified by a proprietary chromatographic technique.

Specification

• Species: EBOV
• Source: E.coli
• Tag: Non
• Description: Ebolavirus (EVD) belongs to the Filoviridae family of proteins which is comprised of a single-strand, non-infectious RNA genome. EVD genome is about 19,000 base pairs long and covers 7 genes in the order 3'-UTR-NP-VP35-VP40-GP-VP30-VP24-L-5'-UTR. There are 4 different ebolaviruses such as: Zaire (EBO-Z), Sudan (EBO-S), Cote d’Ivoire (EBO-CI) and Reston (EBO-R) that differ in amino acid sequence and location of where the gene overlaps. Similar to filoviruses and ebolavirions, EVD is a filamentous element that could appear in 3 forms: shepherd's crook, U shape or a 6 shape. Ebolaviruses may be coiled, toroid, or branched. Most ebolavirions are 80nm wideand 14,000nm long.
• Form: Ebola Zaire protein solution (1.42mg/1ml) is supplied in Phosphate buffer and 0.02% sodium azide.
• Purity: >95% pure as determined by 12% SDS-PAGE (Coomassie blue stain).
• Applications: Immunoassay.
• Stability: Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.

Gene Information

• Gene Name: NP nucleoprotein [ Zaire ebolavirus ]
• Official Symbol: NP
• Synonyms: NP; nucleoprotein
• Gene ID: 911830
• Protein Refseq: NP_066243
• UniProt ID: AAD14590
• Chromosome Location: NC_002549.1 (56..3026)