Recombinant Ebola virus EBOV (subtype Zaire, strain H.sapiens-wt/GIN/2014/Kissidougou-C15) GP protein(Met1-Arg501), His-tagged

• Cat.No.: GP-3318E
• Product Overview: Recombinant Zaire ebolavirus (strain H.sapiens-wt/GIN/2014/Kissidougou-C15) GP (AHX24649.1 ) (Met1-Arg501) was expressed in Insect Cells with a polyhistidine tag at the C-terminus.

Specification

• Species: EBOV
• Source: Insect Cells
• Tag: His
• Protein Length: Met1-Arg501
• Form: Lyophilized from sterile 20mM Tris, 500mM NaCl, 10% glycerol, pH 8.0. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Molecular Mass: The recombinant Zaire ebolavirus (strain H.sapiens-wt/GIN/2014/Kissidougou-C15) GP consists 480 amino acids and predicts a molecular mass of 52.3 kDa.
• Endotoxin: < 1.0 EU per μg protein as determined by the LAL method.
• Purity: > 95 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.