Recombinant E. coli sucC Protein, His-tagged

• Cat.No.: sucC-187E
• Product Overview: Active E. coli (strain K12) C-terminal His-tagged Succinyl-CoA synthetase enzyme purified from E. coli.

Specification

• Species: E.coli
• Source: E.coli
• Tag: His
• Description: Succinyl-CoA synthetase from E. coli is responsible for the coupled hydrolysis of succinyl-CoA to the production of ATP (or GTP) in the substrate-level phosphorylation step of the citric acid cycle (TCA). This enzyme complex is formed as a heterotetramer, α2β2, where the two α subunits each contain the coenzyme A and phosphate binding sites, and the two β subunits each contain the succinate binding sites and provide nucleotide binding specificity. This enzyme is Mg2+-dependent.
• Molecular Mass: 29.8 kDa (α), 41.4 kDa (β)
• Purity: ≥85% estimated by SDS-PAGE
• Unit Definition: One unit is defined as the amount of enzyme required to produce 1 nmol NADH per minute at 25 centigrade in 34 mM Glycylglycine buffer, pH 8.4, 3.4 mM Magnesium Chloride, 1.2 mM ATP, 0.89 mM Coenzyme A, 5.8 mM Succinic acid, 4.1 U/mL pyruvate kinase, 3.4 U/mL Lactic Dehydrogenase.
• Stability: ≥ 1 year
• Storage: At -80 centigrade (as supplied); avoid freeze/thaw cycles by storing protein in aliquots
• Storage Buffer: 50 mM HEPES, pH 8.0, 150 mM sodium chloride, 20% glycerol

Gene Information

• Gene Name: sucC succinyl-CoA synthetase subunit beta [ Escherichia coli str. K-12 substr. MG1655 ]
• Official Symbol: sucC
• Synonyms: sucC; succinyl-CoA synthetase subunit beta; ECK0716; succinyl-CoA synthetase subunit beta; EC 6.2.1.5
• Gene ID: 945312
• Protein Refseq: NP_415256
• UniProt ID: P0A836