Recombinant Dog IL10 protein, His-tagged

• Cat.No.: IL10-492D
• Product Overview: Recombinant Dog IL10 (P48411) aa. (Ser20~IIe181) fused with His tag was produced in HEK293F cells.

Specification

• Species: Dog
• Source: HEK293
• Tag: His
• Protein Length: Ser20~IIe181
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 20.7kDa; Accurate Molecular Mass: 20kDa as determined by SDS-PAGE reducing conditions.
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Purity: > 95%
• Characteristic: Isoelectric point is 9.
• Applications: SDS-PAGE; WB; ELISA; IP; CoIP; EMSA; Reporter Assays; Purification; Amine Reactive Labeling
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Concentration: 200ug/mL
• Storage buffer: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 5% Trehalose and Proclin300.
• Reconstitution: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex

Gene Information

• Gene Name: IL10 interleukin 10 [ Canis lupus familiaris (dog) ]
• Official Symbol: IL10
• Synonyms: IL10; interleukin 10; IL-10; interleukin-10; CSIF; cytokine synthesis inhibitory factor
• Gene ID: 403628
• mRNA Refseq: NM_001003077.1
• Protein Refseq: NP_001003077.1
• UniProt ID: P48411